Additional file1: of Small RNA profiling of low biomass samples: identification and removal of contaminants

Autor: Heintz-Buschart, Anna, Yusuf, Dilmurat, Kaysen, Anne, Etheridge, Alton, JoĂŤlle Fritz, May, Patrick, Beaufort, Carine De, Bimal Upadhyaya, Anubrata Ghosal, Galas, David, Wilmes, Paul
Rok vydání: 2018
DOI: 10.6084/m9.figshare.6268769.v1
Popis: Figure S1. Scheme summarising the different control experiments, the titration experiments and their outcomes. a) Tracing non-human sRNA sequences to contaminants on spin columns by variation of different steps in the isolation protocol and analysis by qPCR assays. Modifications to the steps named at the top are listed below the workflow and the outcomes are summarised at the right hand side. b) Workflow of the titration experiment to determine a minimal safe input volume for all contaminant sequences. UCP column ultra-clean column. (PDF 86Â kb)
Databáze: OpenAIRE