PD-L1 Multiplex and Quantitative Image Analysis for Molecular Diagnostics
| Autor: | Victoria Bingham, Jacqueline James, Matthew P. Humphries, Stephanie G Craig, Stephen McQuaid, Manuel Salto-Tellez, Fatima Abdullahi Sidi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
PD-L1 Cancer Research Pathology medicine.medical_specialty Concordance Immunofluorescence lcsh:RC254-282 Article 03 medical and health sciences 0302 clinical medicine SDG 3 - Good Health and Well-being image analysis otorhinolaryngologic diseases Medicine Multiplex multiplexing biology medicine.diagnostic_test business.industry Macrophage infiltration Molecular diagnostics lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens 030104 developmental biology Oncology 030220 oncology & carcinogenesis biology.protein Immunohistochemistry Non small cell business high-throughput workflow |
| Zdroj: | Cancers, Vol 13, Iss 29, p 29 (2021) Cancers Volume 13 Issue 1 Abdullahi Sidi, F, Bingham, V, Craig, S G, McQuaid, S, James, J, Humphries, M P & Salto-Tellez, M 2020, ' PD-L1 Multiplex and Quantitative Image Analysis for Molecular Diagnostics ', Cancers, vol. 13, no. 1, 29, pp. 1-12 . https://doi.org/10.3390/cancers13010029 |
| ISSN: | 2072-6694 |
| Popis: | Multiplex immunofluorescence (mIF) and digital image analysis (DIA) have transformed the ability to analyse multiple biomarkers. We aimed to validate a clinical workflow for quantifying PD-L1 in non-small cell lung cancer (NSCLC). NSCLC samples were stained with a validated mIF panel. Immunohistochemistry (IHC) was conducted and mIF slides were scanned on an Akoya Vectra Polaris. Scans underwent DIA using QuPath. Single channel immunofluorescence was concordant with single-plex IHC. DIA facilitated quantification of cell types expressing single or multiple phenotypic markers. Considerations for analysis included classifier accuracy, macrophage infiltration, spurious staining, threshold sensitivity by DIA, sensitivity of cell identification in the mIF. Alternative sequential detection of biomarkers by DIA potentially impacted final score. Strong concordance was observed between 3,3&rsquo Diaminobenzidine (DAB) IHC slides and mIF slides (R2 = 0.7323). Comparatively, DIA on DAB IHC was seen to overestimate the PD-L1 score more frequently than on mIF slides. Overall, concordance between DIA on DAB IHC slides and mIF slides was 95%. DIA of mIF slides is rapid, highly comparable to DIA on DAB IHC slides, and enables comprehensive extraction of phenotypic data and specific microenvironmental detail intrinsic to the sample. Exploration of the clinical relevance of mIF in the context of immunotherapy treated cases is warranted. |
| Databáze: | OpenAIRE |
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