Biological and virulence characteristics of Salmonella enterica serovar Typhimurium following deletion of glucose-inhibited division (gidA) gene
| Autor: | Daniel C, Shippy, Nicholas M, Eakley, Philip N, Bochsler, Ashok K, Chopra, Amin A, Fadl |
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| Rok vydání: | 2011 |
| Předmět: |
Salmonella typhimurium
Salmonella Mutant Virulence Mutagenesis (molecular biology technique) medicine.disease_cause Microbiology Mice Bacterial Proteins medicine Animals Intestinal Mucosa Gene Oligonucleotide Array Sequence Analysis Regulation of gene expression Salmonella Infections Animal Mutation biology Reverse Transcriptase Polymerase Chain Reaction Gene Expression Regulation Bacterial biology.organism_classification Mutagenesis Insertional Glucose Infectious Diseases Salmonella enterica Salmonella Infections Female Gene Deletion |
| Zdroj: | Microbial Pathogenesis. 50:303-313 |
| ISSN: | 0882-4010 |
| DOI: | 10.1016/j.micpath.2011.02.004 |
| Popis: | Salmonella enterica serovar Typhimurium is a frequent cause of enteric disease due to the consumption of contaminated food. Identification and characterization of bacterial factors involved in Salmonella pathogenesis would help develop effective strategies for controlling salmonellosis. To investigate the role of glucose-inhibited division gene (gidA) in Salmonella virulence, we constructed a Salmonella mutant strain in which gidA was deleted. Deletion of gidA rendered Salmonella deficient in the invasion of intestinal epithelial cells, bacterial motility, intracellular survival, and induction of cytotoxicity in host cells. Deletion of gidA rendered the organism to display a filamentous morphology compared to the normal rod-shaped nature of Salmonella. Furthermore, a significant attenuation in the induction of inflammatory cytokines and chemokines, histopathological lesions, and systemic infection was observed in mice infected with the gidA mutant. Most importantly, a significant increase in LD(50) was observed in mice infected with the gidA mutant, and mice immunized with the gidA mutant were able to survive a lethal dose of wild-type Salmonella. Additionally, deletion of gidA significantly altered the expression of several bacterial factors associated with pathogenesis as indicated by global transcriptional and proteomic profiling. Taken together, our data indicate GidA as a potential regulator of Salmonella virulence genes. |
| Databáze: | OpenAIRE |
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