Bacteriophage-driven inhibition of biofilm formation in Staphylococcus strains from patients attending a Romanian reference center for infectious diseases
Autor: | Ioana Berciu, Elena Carmina Drăgulescu, Mircea Ioan Popa, Mihaela Oprea, Anca Streinu-Cercel, Dan Oţelea, Mariana Carmen Chifiriuc, Irina Codiţă, Irina Gheorghe, Bleotu Coralia, Marcela Popa, Alina Cristina Neguţ, Oana Săndulescu, Adrian Streinu-Cercel, Olga Mihaela Dorobăţ, Daniela Tălăpan |
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Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
030106 microbiology Biofilm Virulence Biology Staphylococcal infections medicine.disease medicine.disease_cause biology.organism_classification Microbiology In vitro Bacteriophage 03 medical and health sciences 030104 developmental biology Staphylococcus aureus Genetics medicine Molecular Biology Staphylococcus Bacteria |
Zdroj: | FEMS microbiology letters. 363(18) |
ISSN: | 1574-6968 |
Popis: | The increasing burden of invasive biofilm-related staphylococcal infections led to a dire need for new agents to prevent biofilm formation. Bacteriophages may hypothetically alter biofilm through several mechanisms, including induction of depolymerizing enzymes, and lysis of persistent bacteria. We have assessed the influence of commercially-available bacteriophage cocktails on Staphylococcus spp. clinical strains viability and biofilm formation. We analyzed 83 staphylococcal strains from patients consecutively admitted to a Romanian infection reference center from October 2014 through May 2015; the strains were characterized by phenotypic and genetic tools for their resistance and virulence features and for their phyliation. Experiments were performed in triplicate. Methicillin-susceptible strains were significantly more susceptible to all tested phages – 1.7-fold higher susceptibility for PYO, 1.4-fold for INTESTI, 2.9-fold for PHAGYO, 2.7-fold for PHAGESTI and 3.9 for STAPHYLOCOCCAL, respectively; t030 strains were significantly more susceptible to PYO and INTESTI compared with t127 strains. We identified a significant decrease in biofilm formation in the presence of both low and high PYO and INTESTI concentrations ( P < 0.001). In conclusion, Staphylococcus strains from Romania displayed fairly good susceptibility to commercially-available bacteriophages. We have also ascertained the phages - driven in vitro inhibition of biofilm formation, the results potentially impacting prevention of prosthetic infections. |
Databáze: | OpenAIRE |
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