| Autor: |
Nurit Degani-Katzav, Zehavit Shapira, Asaf Grupi, Shimon Yudovich, Shimon Weiss |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| DOI: |
10.5281/zenodo.4704309 |
| Popis: |
Nanodisc technology was implemented as a platform for voltage nanosensors. A FRET-based voltage sensing scheme employing fluorescent nanodiscs and the hydrophobic ion dipicrylamine (DPA) was developed and utilized to optically record membrane potentials on the single nanodisc level. Ensemble- and single- nanosensor recordings were demonstrated for HEK293 cells and primary cortical neuron cells. Conjugation of nanodiscs to anti-GABA-A antibodies allowed for site specific membrane potential measurements from post synaptic sites. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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