A new assay of microbial lipases with emulsified trioleoyl glycerol

Autor:	Nina Peled, Michael C. Krenz
Rok vydání:	1981
Předmět:	Chromatography Biophysics Substrate (chemistry) Cell Biology Lipase Hydrogen-Ion Concentration Biochemistry chemistry.chemical_compound Kinetics chemistry Emulsion Glycerol Methods Organic chemistry Triolein Molecular Biology Rhizopus Olive oil Candida
Zdroj:	Analytical biochemistry. 112(2)
ISSN:	0003-2697
Popis:	An assay procedure for microbial lipases is proposed in which the substrate is an emulsion of triolein (trioleoyl glycerol). The fatty acids released at 30°C are titrated with a pH stat at a pH of 7.7. Although the activity values obtained with this method are lower than those determined with the olive oil method, the results are highly reproducible and the preparation of the substrate is straightforward.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::23b78cf545822da208d221232233ee46 https://pubmed.ncbi.nlm.nih.gov/7258637 Zobrazit plný text záznamu