A novel ladder-like lectin relates to sites of mucosal immunity in Atlantic halibut (Hippoglossus hippoglossus L.)
| Autor: | Bergljót Magnadóttir, Sigrun Lange, Sigridur Gudmundsdottir |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Fish Proteins
0301 basic medicine animal structures Flounder Aquatic Science Halibut 03 medical and health sciences Lectins Animals Environmental Chemistry Amino Acid Sequence Immunity Mucosal Galectin biology Lectin 04 agricultural and veterinary sciences General Medicine Hippoglossus hippoglossus biology.organism_classification Immunohistochemistry Molecular biology Complement system 030104 developmental biology Organ Specificity 040102 fisheries biology.protein 0401 agriculture forestry and fisheries Antibody Ficolin |
| Zdroj: | Fish & Shellfish Immunology. 87:9-12 |
| ISSN: | 1050-4648 |
| DOI: | 10.1016/j.fsi.2018.12.034 |
| Popis: | A novel 27 kDa ladder-lectin-like protein, showing a multimeric structure under non-reducing conditions, was isolated from halibut serum by binding to N-acetyl glucosamine. Mass-spectrometry analysis did not show significant homology with known proteins. Specific antibodies were produced and used in immunohistochemistry on tissue sections of early halibut ontogeny from 119 until 1050 °d post hatching. A strong positive response was detected in the mucosal cells of the skin, gills and gut, indicating a role in the mucosal immune defence at these sites. Further immunopositivity was detected in liver, myeloma of kidney and the brain at different developmental stages but predominant expression was found in mucosal surfaces at later stages of development tested (1050 °d). It is still uncertain whether this ladder-like lectin forms part of the complement pathway, as a lectin or ficolin, or if it belongs to galectins. A strong detection in mucosal surfaces on skin, gills and gut, show similar patterns of expression as both mucosal lectins and galectins in other fish. Detection in neuronal tissue may indicate putative roles in tissue remodelling of brain and in ongoing neurogenesis in the fish eye. |
| Databáze: | OpenAIRE |
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