Removal of acetaminophen in water by laccase immobilized in barium alginate
| Autor: | Supot Punbut, Karnika Ratanapongleka |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
Alginates Sodium chemistry.chemical_element Portable water purification 010501 environmental sciences 01 natural sciences Water Purification chemistry.chemical_compound Glucuronic Acid 010608 biotechnology Environmental Chemistry Waste Management and Disposal 0105 earth and related environmental sciences Water Science and Technology Acetaminophen Laccase Chromatography Aqueous solution biology Hexuronic Acids digestive oral and skin physiology Barium chloride Substrate (chemistry) Water General Medicine Hydrogen-Ion Concentration Glucuronic acid Enzymes Immobilized digestive system diseases Enzyme assay Kinetics chemistry Models Chemical biology.protein Water Pollutants Chemical |
| Zdroj: | Environmental technology. 39(3) |
| ISSN: | 1479-487X |
| Popis: | This research has focused on the optimization of immobilized laccase condition and utilization in degradation of acetaminophen contaminated in aqueous solution. Laccase from Lentinus polychrous was immobilized in barium alginate. The effects of laccase immobilization such as sodium alginate concentration, barium chloride concentration and gelation time were studied. The optimal conditions for immobilization were sodium alginate 5% (w/v), barium chloride 5% (w/v) and gelation time of 60 min. Immobilized laccase was then used for acetaminophen removal. Acetaminophen was removed quickly in the first 50 min. The degradation rate and percentage of removal increased when the enzyme concentration increased. Immobilized laccase at 0.57 U/g-alginate showed the maximum removal at 94% in 240 min. The removal efficiency decreased with increasing initial acetaminophen concentration. The Km value for immobilized laccase (98.86 µM) was lower than that of free laccase (203.56 µM), indicating that substrate affini... |
| Databáze: | OpenAIRE |
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