Changes in mitochondrial function in porcine vitrified MII-stage oocytes and their impacts on apoptosis and developmental ability
| Autor: | Rong Rui, Caifeng Wu, Jianjun Dai, Yingfang Niu, Caroline W. Muneri, Shushan Zhang, Zhang Defu |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Swine
SOD1 Parthenogenesis MFN2 Apoptosis Biology Mitochondrion General Biochemistry Genetics and Molecular Biology Cryopreservation Andrology Adenosine Triphosphate Annexin Botany Animals Vitrification chemistry.chemical_classification Membrane Potential Mitochondrial Reactive oxygen species General Medicine Mitochondria chemistry Oocytes Female General Agricultural and Biological Sciences Reactive Oxygen Species |
| Zdroj: | Cryobiology. 71(2) |
| ISSN: | 1090-2392 |
| Popis: | The purpose of this study was to investigate the changes in mitochondria in porcine MII-stage oocytes after open pulled straw (OPS) vitrification and to determine their roles in apoptosis and in vitro developmental ability. The mitochondrial membrane potential (ΔΨm), reactive oxygen species (ROS) level, adenosine-5'-triphosphate (ATP) concentration, mitochondrial distribution, mitochondrial ultrastructure, early-stage apoptosis with Annexin V-FITC staining, survival rate, parthenogenetic developmental ability and related gene expression were measured in the present experiments. The results showed that: (1) the mitochondrial ΔΨm of vitrified-thawed oocytes (1.05) was lower than that of fresh oocytes 1.24 (P |
| Databáze: | OpenAIRE |
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