High-performance liquid chromatographic method for the determination of mycophenolate mofetil in human plasma
| Autor: | Rosy Lee, Martin Kaloostian, Thomas Tarnowski, Irene Tsina, Belinda Wong |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Quality Control
Chromatography Chemistry Elution General Chemistry Organ Transplantation Hydrogen-Ion Concentration Mycophenolic Acid Mycophenolate High-performance liquid chromatography Sensitivity and Specificity Mycophenolic acid Kinetics Drug Stability Blood plasma medicine Humans Particle size Solid phase extraction Quantitative analysis (chemistry) Chromatography High Pressure Liquid Immunosuppressive Agents medicine.drug |
| Zdroj: | Journal of chromatography. B, Biomedical applications. 681(2) |
| ISSN: | 1572-6495 |
| Popis: | A method for the quantification of mycophenolate mofetil (MMF, CellCept) in plasma using solid-phase extraction and HPLC is described here. A solution of internal standard is added to a 0.5-ml plasma aliquot. The resulting sample is treated with water and dilute HCl and applied to a C18 solid-phase extraction column. After a water wash, the MMF and internal standard are eluted with methanol-0.1 M citrate-phosphate buffer, pH 2.6 (80:20, v/v). A 20-microliters aliquot of the eluate is injected onto a C18 column (5 microns particle size, 150 x 4.6 mm I.D.) and eluted at ambient temperature with acetonitrile-0.05 M citrate-phosphate buffer, pH 3.6, containing 0.02 M heptanesulfonic acid (41:59, v/v). Quantification is achieved by UV detection at 254 nm. The method is reproducible, accurate and specific for MMF. Using 0.5 ml of plasma for analysis, the quantification limit is 0.400 microgram/ml and the range is 0.400-20 micrograms/ml. Based on the stability profile of MMF in plasma, it is recommended that blood samples collected following intravenous infusion be immediately stored on ice and that plasma be prepared rapidly, immediately stored frozen at -80 degrees C and analyzed within four months of collection. |
| Databáze: | OpenAIRE |
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