Recruitment of γδ T cells to the lesion via the CCL2/CCR2 signaling after spinal cord injury
| Autor: | Hao-Ran Zhu, Cheng-Hong Sun, Minmin Chang, Si-Tao Li, Cheng Zhong, Wen-Cai Zhang, Jing-Chun Yao, Zhizhong Li, Ping Xu, Guodong Sun, Feng Zhang |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
CCR2 Chemokine Receptors CCR2 T cell T-Lymphocytes Immunology Spinal cord injury CCL2 lcsh:RC346-429 03 medical and health sciences Cellular and Molecular Neuroscience Chemokine receptor Mice 0302 clinical medicine Immune system medicine Animals Humans Neuroinflammation lcsh:Neurology. Diseases of the nervous system Chemokine CCL2 Spinal Cord Injuries Inflammation γδ T cell Mice Knockout biology business.industry General Neuroscience Research Receptors Antigen T-Cell gamma-delta medicine.disease Mice Inbred C57BL Chemotaxis Leukocyte 030104 developmental biology medicine.anatomical_structure Neurology biology.protein business 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Journal of Neuroinflammation, Vol 18, Iss 1, Pp 1-16 (2021) Journal of Neuroinflammation |
| ISSN: | 1742-2094 |
| Popis: | Background Immune cell infiltration and neuroinflammation are heavily associated with spinal cord injury (SCI). C-C motif chemokine ligand 2/C-C chemokine receptor type 2 (CCL2/CCR2) axis has been identified as a critical role player during the invasion of immune cells to lesions in many diseases. γδ T cells, a subgroup of T cells, manage the course of inflammation response in various diseases; however, it remains unknown whether γδ T cells are recruited to injury site through CCL2/CCR2 signaling and exert the regulation effect on neuroinflammation after SCI. Methods Basso Mouse Scale (BMS), regularity index, cadence, max contact area, and motor-evoked potential testing (MEP) were measured to determine the neurological function recovery after spinal cord injury. Nissl staining was performed to identify the number of surviving motor neurons at lesion epicenter. Immunofluorescence, Western blot, enzyme-linked immunosorbent assays (ELISA), and quantitative real-time polymerase chain reaction (QRT-PCR) also were employed to evaluate the expression of associated proteins and genes. Results In this study, we demonstrated that TCRδ−/− mice present improved neurological recovery after SCI. γδ T cell recruitment to the SCI site was significantly reduced and motor functional improvement enhanced in CCL2−/− and CCR2−/− mouse strains. Furthermore, reconstitution of TCRδ−/− mice with γδ T cells extracted from CCR2−/− mice also showed similar results to CCL2 and CCR2 deficient mice. Conclusions In conclusion, γδ T cell recruitment to SCI site promotes inflammatory response and exacerbates neurological impairment. CCL2/CCR2 signaling is a vital recruitment mechanism of γδ T cells to the SCI site, and it may be taken as a novel therapeutic target for future SCI. |
| Databáze: | OpenAIRE |
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