Glycogen Content Regulates Peroxisome Proliferator Activated Receptor-∂ (PPAR-∂) Activity in Rat Skeletal Muscle
| Autor: | Keith Baar, Micah Y. Belew, Angela Papalamprou, Alan Corstorphine, Andrew Philp, Matthew G. MacKenzie, Mhairi C. Towler, D. Grahame Hardie |
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| Přispěvatelé: | Bassaganya-Riera, Josep |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
General Science & Technology Science 1.1 Normal biological development and functioning Peroxisome Proliferator-Activated Receptors Peroxisome proliferator-activated receptor Biology AMP-Activated Protein Kinases Cell Line chemistry.chemical_compound Rare Diseases Endurance training Underpinning research Internal medicine Physical Conditioning Animal medicine Genetics Animals Kinase activity Muscle Skeletal Nutrition chemistry.chemical_classification Multidisciplinary Glycogen Myogenesis Animal AMPK Skeletal muscle Skeletal Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha Physical Conditioning Rats Enzyme Activation Endocrinology medicine.anatomical_structure Glucose chemistry Medicine Muscle Female medicine.symptom Muscle contraction Research Article Transcription Factors |
| Zdroj: | PLoS ONE PloS one, vol 8, iss 10 PLoS ONE, Vol 8, Iss 10, p e77200 (2013) |
| ISSN: | 1932-6203 |
| Popis: | Performing exercise in a glycogen depleted state increases skeletal muscle lipid utilization and the transcription of genes regulating mitochondrial β-oxidation. Potential candidates for glycogen-mediated metabolic adaptation are the peroxisome proliferator activated receptor (PPAR) coactivator-1α (PGC-1α) and the transcription factor/nuclear receptor PPAR-∂. It was therefore the aim of the present study to examine whether acute exercise with or without glycogen manipulation affects PGC-1α and PPAR-∂ function in rodent skeletal muscle. Twenty female Wistar rats were randomly assigned to 5 experimental groups (n = 4): control [CON]; normal glycogen control [NG-C]; normal glycogen exercise [NG-E]; low glycogen control [LG-C]; and low glycogen exercise [LG-E]). Gastrocnemius (GTN) muscles were collected immediately following exercise and analyzed for glycogen content, PPAR-∂ activity via chromatin immunoprecipitation (ChIP) assays, AMPK α1/α2 kinase activity, and the localization of AMPK and PGC-1α. Exercise reduced muscle glycogen by 47 and 75% relative to CON in the NG-E and LG-E groups, respectively. Exercise that started with low glycogen (LG-E) finished with higher AMPK-α2 activity (147%, p |
| Databáze: | OpenAIRE |
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