Evidence for a Colonic PAF Receptor
| Autor: | Jeffrey D. Carter, Donald L. Kaminski, Michael L. Niehoff, Anil D. Kulkarni, John W. Standeven, Y.G. Deshpande, Walter E. Longo, Anthony M. Vernava, Gregory Polites, Bhugol Chandel |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
medicine.medical_specialty
Colon Thromboxane medicine.medical_treatment Receptors Cell Surface Platelet Membrane Glycoproteins In Vitro Techniques Inferior mesenteric artery Receptors G-Protein-Coupled chemistry.chemical_compound Internal medicine medicine.artery medicine Animals Platelet Activating Factor Receptor Prostanoid Azepines Triazoles respiratory system Endocrinology Alprazolam Eicosanoid chemistry Eicosanoids Inferior mesenteric vein lipids (amino acids peptides and proteins) Surgery Rabbits medicine.drug Prostaglandin E |
| Zdroj: | Journal of Surgical Research. 58:12-18 |
| ISSN: | 0022-4804 |
| DOI: | 10.1006/jsre.1995.1003 |
| Popis: | We sought to determine the effect of exogenously administered platelet-activating factor (PAF) on eicosanoid release from the left colon of the rabbit. Using an isolated buffer-perfused rabbit left colon preparation, 1.0- or 5.0-μg doses of PAF were infused into the inferior mesenteric artery. Effluents from the inferior mesenteric vein and colonic lumen were collected and the concentrations of the eicosanoids, prostaglandin E, 6-ketoprostaglandin F 1α , thromboxane B 2 , and leukotriene B 4 (LTB 4 ), were measured by ELISA. During PAF infusion there was a significant increase of all prostanoids, but not LTB 4 into the venous effluent and colonic luminal perfusate when compared to control experiments. Additional studies were performed by pretreating the colons with the PAF antagonists WEB-2170 or alprazolam prior to PAF infusion. Both venous and luminal effluent prostanoid release was effectively blocked by WEB-2170, but not by alprazolam. Colons pretreated with WEB-2170 prior to PAF had markedly diminished tissue injury when compared to colons treated with PAF alone. Inhibition of PAF-stimulated prostanoid release by WEB-2170 suggests that a PAF-sensitive receptor is present in rabbit colonic tissue which may induce eicosanoid-mediated tissue injury. |
| Databáze: | OpenAIRE |
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