OMNIgene SPUTUM: A good transport and decontaminating reagent for tuberculosis testing
| Autor: | Prince Asare, Emelia Danso, Kenneth Mawuta Hayibor, Pius Mawutor Klevor, Osei-Wusu Stephen, Samuel Yaw Aboagye, Adwoa Asante-Poku, Dorothy Yeboah-Manu, Diana Asema Asandem |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) sputum decontamination medicine.medical_specialty Tuberculosis 030106 microbiology lcsh:QR1-502 Transportation Ghana Sensitivity and Specificity lcsh:Microbiology Sputum culture Specimen Handling Agar plate 03 medical and health sciences 0302 clinical medicine fluids and secretions Refrigeration Internal medicine Contaminants medicine Humans 030212 general & internal medicine Controlled experiment General hospital Decontamination Bacteriological Techniques medicine.diagnostic_test business.industry Sputum Mycobacterium tuberculosis medicine.disease Laboratories Hospital Infectious Diseases Specimen Quality tuberculosis Reagent Indicators and Reagents OMNIgene SPUTUM medicine.symptom business sputum transport |
| Zdroj: | International Journal of Mycobacteriology, Vol 7, Iss 3, Pp 222-227 (2018) |
| ISSN: | 2212-5531 |
| Popis: | Background: Sputum culture is limited to centralized facilities. Thus, samples require transportation from peripheral laboratories to these facilities, compromising specimen quality since it is difficult to maintain cold chain. We evaluated OMNIgene SPUTUM Reagent (OMS) for transporting sputum samples for tuberculosis (TB) testing. The study was carried out at Noguchi Memorial Institute for Medical Research using sputa from Korle Bu Teaching Hospital and La General Hospital in Ghana. Methods: In a laboratory-based controlled experiment (CE), sputum contaminants were determined on blood agar before treatment with OMS and N-acetyl-L-cysteine-sodium hydroxide (NALC-NaOH). TB testing included smear microscopy, culture, and Xpert MTB/RIF. Afterward, two peripheral laboratories were trained to transport sputum samples with OMS without cold chain. Positivity, negativity, and contamination rates were compared between both methods using Chi-square and Fisher's exact tests. Cohen's Kappa was also used to determine agreements. Results: Among 104 sputum samples analyzed in the CE, 93 (89.4%) had bacterial growth on blood agar before decontamination, while 6 (5.8%) and 5 (4.8%) contaminated after NALC-NaOH and OMS treatment, respectively. Contamination was high with NALC-NaOH (12.8%) than OMS (4.3%) on Lowenstein–Jensen media (P < 0.001), but mycobacterial positivity was comparable: NALC-NaOH of 74.5% and OMS of 78.7%. Smear positivity after NALC-NaOH treatment was 89.4% and OMS was 75.9% (P = 0.491). All except one of the samples tested positive by Xpert MTB/RIF after both treatment. Sixteen samples were evaluated in the field experiment and 81.3% yielded positive culture, and no contamination on LJ was observed. Conclusion: Our findings indicate that OMS works well as a transport and decontaminating reagent of samples for TB testing. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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