Antibacterial activity of glycosylated and phosphorylated chromogranin A-derived peptide 173-194 from bovine adrenal medullary chromaffin granules
| Autor: | Jean-Marc Strub, Marie-Hélène Metz-Boutigue, Dominique Aunis, Yannick Goumon, Alain Van Dorsselaer, Calliope Capon, Karine Lugardon, Michel Lopez, Marc Moniatte |
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| Rok vydání: | 1996 |
| Předmět: |
endocrine system
Glycosylation Molecular Sequence Data Peptide macromolecular substances Biochemistry chemistry.chemical_compound Chromogranins Animals Chromaffin Granules Amino Acid Sequence Phosphorylation Molecular Biology Peptide sequence Chromatography High Pressure Liquid chemistry.chemical_classification biology Chromogranin A Tyrosine phosphorylation Cell Biology biology.organism_classification Peptide Fragments Anti-Bacterial Agents chemistry Adrenal Medulla Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization biology.protein Cattle Antibacterial activity Bacteria |
| Zdroj: | The Journal of biological chemistry. 271(45) |
| ISSN: | 0021-9258 |
| Popis: | Recently, we have isolated from bovine chromaffin granules and identified two natural peptides possessing antibacterial activity: secretolytin (chromogranin B 614-626) and enkelytin (proenkephalin-A 209-237). Here, we characterize a large natural fragment, corresponding to chromogranin A 79-431, that inhibits growth of both Gram-positive and Gram-negative bacteria. The aim of the present work was to determine the shortest active peptide located in the 79-431 chromogranin A region. Three peptides, which shared the same 173-194 chromogranin A sequence (YPGPQAKEDSEGPSQGPASREK) but differed in post-translational modifications, including O-glycosylation and tyrosine phosphorylation, were isolated. A detailed study using microsequencing and mass spectrometry allowed us to correlate their antibacterial activity with these post-translational modifications. The chromogranin A precursor fragment (79-431) and the active glycosylated and phosphorylated peptides were, respectively, named prochromacin and chromacin (P, G, and PG for phosphorylated, glycosylated, and phosphorylated-glycosylated form). |
| Databáze: | OpenAIRE |
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