< Omega >-3 PUFA Attenuates LPS-Induced Neuro-Injury of Neonatal Rats through the PI3K/AKT Pathway
| Autor: | Ji-peng Shi, Jing Liu, Wei Fu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Lipopolysaccharides
Male 0301 basic medicine medicine.medical_specialty Apoptosis Hippocampus PC12 Cells Neuroprotection Rats Sprague-Dawley Phosphatidylinositol 3-Kinases 03 medical and health sciences 0302 clinical medicine Downregulation and upregulation Cell Movement Internal medicine Fatty Acids Omega-3 medicine Animals Protein kinase B PI3K/AKT/mTOR pathway Cell Proliferation Neurons TUNEL assay Chemistry Akt/PKB signaling pathway General Neuroscience Rats Neuroprotective Agents 030104 developmental biology Endocrinology Animals Newborn Phosphorylation lipids (amino acids peptides and proteins) Proto-Oncogene Proteins c-akt 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | Neuroscience. 414:112-127 |
| ISSN: | 0306-4522 |
| Popis: | Inflammation may result in periventricular leukomalacia, which is the leading cause of preterm brain encephalopathy. Moreover,omega-3 polyunsaturated fatty acids (omega-3 PUFAs) play a pivotal role against central nervous system injury, which is likely related to its anti-inflammatory effect. However, the mechanism regarding the remedial effects ofomega-3 PUFA for LPS-induced neuro-injury has remained unclear. In this study, newborn SD rats were intraperitoneally injected with LPS or omega-3 PUFA, and the proliferation and apoptosis of neurocytes in the hippocampus were measured by TUNEL and BrdU. Quantitative real-time PCR (qPCR) and Western blot assay were used to analyze the mRNA and protein levels of PI3K, AKT and β-catenin in vitro and in vivo. We found thatomega-3 PUFA promoted the proliferation and migration of neurocytes in vitro and in vivo and inhibited apoptosis. Furthermore, we confirmed thatomega-3 PUFA through the PI3K/AKT signaling pathway positively regulated the expression of PI3K and further caused the phosphorylation of AKT activation, followed by the upregulation of β-catenin expression. Interestingly, this phenomenon became more noticeable with the combined application ofomega-3 PUFA and a PI3K/AKT agonist. In conclusion, we confirm thatomega-3 PUFA plays an important role in neuroprotection by activating the PI3K/AKT/β-catenin pathway. It may be a promising strategy against brain injury. |
| Databáze: | OpenAIRE |
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