EphB2 contributes to human naive B‐cell activation and is regulated by miR‐185
| Autor: | Qi Long, Min Wang, Miao Yu, Jing Yuan, Wei Liang, Ming-Xin Zhu, Yuhua Liao, Huan-Huan Li, Tong Zhao, Xiang Cheng, Shuang Wen |
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| Rok vydání: | 2014 |
| Předmět: |
Receptor
EphB2 Naive B cell Ephrin-B2 Ephrin-B1 Lymphocyte Activation Biochemistry Receptor tyrosine kinase Genetics medicine Humans Secretion RNA Messenger RNA Small Interfering Receptor Notch1 Molecular Biology B-Lymphocytes biology Tumor Necrosis Factor-alpha Chemistry Monocyte Molecular biology MicroRNAs medicine.anatomical_structure Immunoglobulin M Immunoglobulin G biology.protein RNA Interference Cytokine secretion Tumor necrosis factor alpha Antibody Tyrosine kinase Signal Transduction Biotechnology |
| Zdroj: | The FASEB Journal. 28:3609-3617 |
| ISSN: | 1530-6860 0892-6638 |
| Popis: | EphB2 is an important member of the receptor tyrosine kinases. Recently, EphB2 was shown to facilitate T-cell migration and monocyte activation. However, the effects of EphB2 on B cells remain unknown. In this study, the expression of EphB2 on B cells was tested by Western blot, and the roles of EphB2 in B-cell proliferation, cytokine secretion, and immunoglobulin (Ig) production were evaluated using EphB2 siRNA interference in human B cells from healthy volunteers. Our study revealed that EphB2 was distributed on naive B cells and was up-regulated on activated B cells. Moreover, B-cell proliferation (decreased by 22%, P |
| Databáze: | OpenAIRE |
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