Estimating bacteria diversity in different organs of nine species of mosquito by next generation sequencing
Autor: | Irene Ricci, Jovana Bozic, Paolo Rossi, Alessia Cappelli, Matteo Valzano, E Nunzi, Roberta Spaccapelo, R Catanzani, Aurelio Serrao, Claudia Damiani, Mv Mancini, M Tallarita, Guido Favia, Anastasia Accoti |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
DNA
Bacterial Male 0301 basic medicine Microbiology (medical) TRANSMISSION 030106 microbiology lcsh:QR1-502 Biology Microbiology Mosquitoes DNA sequencing lcsh:Microbiology 03 medical and health sciences Symbiosis RNA Ribosomal 16S SYMBIONT Animals Metagenomics Symbionts GUT INSECT ENDOSYMBIONTS MICROBIOTA DROSOPHILA MUTUALISM LARVAL Bacteria Transmission (medicine) Host (biology) Animal Structures High-Throughput Nucleotide Sequencing Biodiversity Holobiont Culicidae 030104 developmental biology Evolutionary biology Vector (epidemiology) Female Adaptation Research Article |
Zdroj: | BMC Microbiology, Vol 18, Iss 1, Pp 1-10 (2018) BMC Microbiology |
Popis: | Background Symbiosis in insects is accumulating significant amount of studies: the description of a wide array of mutualistic associations across the evolutionary history of insects suggests that resident microbiota acts as a driving force by affecting several aspects of hosts biology. Among arthropods, mosquito midgut microbiota has been largely investigated, providing crucial insights on the role and implications of host-symbiont relationships. However, limited amount of studies addressed their efforts on the investigation of microbiota colonizing salivary glands and reproductive tracts, crucial organs for pathogen invasion and vertical transmission of symbiotic microorganisms. Using 16S rRNA gene sequencing-based approach, we analysed the microbiota of gut, salivary glands and reproductive tracts of several mosquito species, representing some of the main vectors of diseases, aiming at describing the dynamics of bacterial communities within the individual. Results We identified a shared core microbiota between different mosquito species, although interesting inter- and intra-species differences were detected. Additionally, our results showed deep divergences between genera, underlining microbiota specificity and adaptation to their host. Conclusions The comprehensive landscape of the bacterial microbiota components may ultimately provide crucial insights and novel targets for possible application of symbionts in innovative strategies for the control of vector borne diseases, globally named Symbiotic Control (SC), and suggesting that the holobiont of different mosquito species may significantly vary. Moreover, mosquito species are characterized by distinctive microbiota in different organs, likely reflecting different functions and/or adaptation processes. Electronic supplementary material The online version of this article (10.1186/s12866-018-1266-9) contains supplementary material, which is available to authorized users. |
Databáze: | OpenAIRE |
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