Early hypothalamic activation of combined fos and CRH41 immunoreactivity and of CRH41 release in push-pull cannulated rats after systemic endotoxin challenge
| Autor: | Givalois, G, Siaud, P., Mekaouche, M., Ixart, G., Malaval, F., Assenmacher, I., Barbanel, Gérard, Givalois, Laurent |
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| Přispěvatelé: | Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Mécanismes moléculaires dans les démences neurodégénératives (MMDN), Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL) |
| Jazyk: | angličtina |
| Rok vydání: | 1995 |
| Předmět: |
Lipopolysaccharides
Male medicine.medical_specialty Lipopolysaccharide Corticotropin-Releasing Hormone Stimulation Push–pull perfusion Catheterization Rats Sprague-Dawley 03 medical and health sciences Immunolabeling chemistry.chemical_compound 0302 clinical medicine Adrenocorticotropic Hormone Internal medicine medicine Colchicine Animals Molecular Biology 030304 developmental biology Neurons 0303 health sciences Chemistry General Neuroscience Immunohistochemistry 3. Good health Rats Endocrinology Hypothalamus Median eminence Tumor necrosis factor alpha [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC] Neurology (clinical) Proto-Oncogene Proteins c-fos 030217 neurology & neurosurgery Paraventricular Hypothalamic Nucleus |
| Zdroj: | Molecular and Chemical Neuropathology Molecular and Chemical Neuropathology, 1995, 26 (2), pp.171-186. ⟨10.1007/BF02815011⟩ |
| ISSN: | 1044-7393 |
| DOI: | 10.1007/BF02815011⟩ |
| Popis: | International audience; We previously showed that intra-arterial endotoxin infusion (lipopolysaccharide [LPS]: 25 micrograms.kg-1) induced an early (15 min) and sustained (480 min) rise in plasma ACTH associated with delayed (60-120 min) increases in plasma concentrations of TNF alpha, IL-6, and IL-1 beta. In the present study, we followed the post-LPS time-course of immunocytochemical expression of Fos-like activity in CRH41 neurons whose immunolabeling was enhanced by icv colchicine pretreatment 48 h before the LPS, and CRH41 release in the push-pull cannulated median eminence of free-moving rats, in parallel with the ACTH response. The earliest Fos-like activity in IR-CHR41 neurons was detected 30 min post-LPS. Colchicine strongly inhibited the LPS-induced activation of Fos expression in single-labeled paraventricular neurons. CRH41 release in the median eminence displayed a biphasic stimulation pattern, with a first peak (+60%) at 15 min together with the ACTH surge, followed by a second rise beginning at 45 min and lasting more than 2 h. Thus, the early stage of the ACTH surge following a nonlethal endotoxin challenge (< 60 min) already involves the activation of CRH41-producing neurons. |
| Databáze: | OpenAIRE |
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