Distribution of gene segments of the pandemic A(H1N1) 2009 virus lineage in pig populations
| Autor: | Kosuke Okuya, Makoto Ozawa, T. Furuya, F. Koike, Toshiko Kawabata, S. Akimoto, A. Taneno, Eisaburo Deguchi, Aya Matsuu, M. Ito |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Lineage (genetic) Swine animal diseases viruses Neuraminidase Genome Viral Biology Virus Madin Darby Canine Kidney Cells 03 medical and health sciences Viral Proteins Dogs Influenza A Virus H1N1 Subtype Japan Orthomyxoviridae Infections Influenza A Virus H1N2 Subtype Pandemic Pig farming Animals Humans Pig farms Gene Pandemics Phylogeny Swine Diseases General Veterinary General Immunology and Microbiology Influenza A Virus H3N2 Subtype virus diseases General Medicine Virology 030104 developmental biology biology.protein |
| Zdroj: | Transboundary and emerging diseases. 65(6) |
| ISSN: | 1865-1682 |
| Popis: | Swine influenza viruses (SIVs) are important not only for pig farming, but also for public health. In fact, pandemic A(H1N1) 2009 viruses [A(H1N1)pdm09] were derived from SIVs. Therefore, timely characterization of locally circulating SIVs is necessary for understanding the global status of SIVs. To genetically characterize SIVs circulating in Japanese pig populations, we isolated 24 SIVs of three subtypes (17 H1N1, four H1N2 and three H3N2 strains) from 14 pig farms in Japan from 2013 to 2016. Genetic analyses revealed that the haemagglutinin (HA) and neuraminidase (NA) genes of the 17 H1N1 and the HA gene of one H1N2, A/swine/Aichi/02/2016 (H1N2), SIVs belonged to the A(H1N1)pdm09 lineage. More importantly, all of the remaining six gene segments (i.e., PB1, PB1, PA, NP, M and NS) of the 24 SIVs, regardless of the HA and NA subtype, were also classified as belonging to the A(H1N1)pdm09 lineage. These results indicate that gene segments of A(H1N1)pdm09 lineage are widely distributed in SIVs circulating in Japanese pig populations In addition, the NA gene of A/swine/Aichi/02/2016 (H1N2) shared less than 88.5% nucleotide identity with that of the closest relative A/swine/Miyagi/5/2003 (H1N2), which was isolated in Japan in 2003. These results indicate the sustained circulation of classical H1N2-derived SIVs with remarkable diversity in the NA genes in Japanese pig populations. These findings highlight the necessity of both intensive biosecurity systems and active SIV surveillance in pig populations worldwide for both animal and public health. |
| Databáze: | OpenAIRE |
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