Role of CD4 and CD8 T-lymphocytes, B-lymphocytes and Natural Killer cells in the prediction of radiation-induced late toxicity in cervical cancer patients
| Autor: | Elisa Bordón, Pedro C. Lara, Beatriz Pinar, Carlos Rodríguez-Gallego, Marta Lloret, Luis Alberto Henríquez-Hernández |
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| Rok vydání: | 2010 |
| Předmět: |
Programmed cell death
Pathology medicine.medical_specialty Cell Survival medicine.medical_treatment Uterine Cervical Neoplasms Biology Sensitivity and Specificity Bone cell medicine Humans Cytotoxic T cell Radiology Nuclear Medicine and imaging Lymphocytes Radiation Injuries Cells Cultured Radiological and Ultrasound Technology Reproducibility of Results Radiation therapy Treatment Outcome Apoptosis Peripheral blood lymphocyte Toxicity Cancer research Biological Assay Female Radiotherapy Conformal CD8 |
| Zdroj: | International Journal of Radiation Biology. 87:424-431 |
| ISSN: | 1362-3095 0955-3002 |
| Popis: | To analyse the role of in vitro radio-induced apoptosis of lymphocyte subpopulations as predictive test for late effects in cervical cancer patients treated with radiotherapy.Ninety-four consecutive patients and four healthy controls were included in the study. Toxicity was evaluated using the Late Effects Normal Tissue-Subjective, Objective, Management, and Analytic (LENT-SOMA) scale. Peripheral blood lymphocyte subpopulations were isolated and irradiated at 0, 1, 2 and 8 Gy, and then collected 24, 48 and 72 h after irradiation. Apoptosis was measured by flow cytometry.Radiation-induced apoptosis increased with radiation dose and time of incubation, and data fitted to a semi-logarithmic model defined by two constants: α (percentage of spontaneous cell death) and β (percentage of cell death induced at a determined radiation dose). Higher β values in cytotoxic T-lymphocytes (CD8) and bone cells (B-lymphocytes) were observed in patients with low bowel toxicity (hazard ratio (HR) = 0.96, p = 0.002 for B-cells); low rectal toxicity (HR = 0.96, p = 0.020; HR = 0.93, p = 0.05 for B and CD8 subpopulations respectively); low urinary toxicity (HR = 0.93, p = 0.003 for B-cells) and low sexual toxicity (HR = 0.93, p = 0.010 for CD8-cells).Radiation-induced CD8 T-lymphocytes and, for the first time, B-lymphocytes apoptosis can predict differences in late toxicity in cervical cancer patients. |
| Databáze: | OpenAIRE |
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