An efficient enzymatic synthesis of thiamin pyrophosphate

Autor:	Cynthia Kinsland, Tadhg P. Begley, Jonathan S. Melnick, Jason J. Reddick, K.Ingrid Sprinz
Rok vydání:	2003
Předmět:	Pyrimidine Stereochemistry Clinical Biochemistry Pharmaceutical Science Biochemistry Polymerase Chain Reaction chemistry.chemical_compound Biosynthesis Drug Discovery Escherichia coli Cloning Molecular Thiamin pyrophosphate Thiazole Molecular Biology DNA Primers Phosphotransferases (Phosphate Group Acceptor) Base Sequence Kinase Organic Chemistry food and beverages Recombinant Proteins B vitamins chemistry Yield (chemistry) Molecular Medicine Thiamine Thiamine Pyrophosphate human activities
Zdroj:	Bioorganicmedicinal chemistry letters. 13(22)
ISSN:	0960-894X
Popis:	Thiamin pyrophosphate was synthesized in 71% yield, on a multi-milligram scale, using overexpressed thiazole kinase, pyrimidine kinase, thiamin phosphate synthase, and thiamin phosphate kinase. This provides a facile route to isotopically labeled thiamin pyrophosphate from its readily available pyrimidine and thiazole precursors.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2399942243d50e7958f10ef28dbc2b31 https://pubmed.ncbi.nlm.nih.gov/14592524 Zobrazit plný text záznamu Full Text from ScienceDirect