Chromosomal aberrations in large Japanese field mice (Apodemus speciosus) captured near Fukushima Dai-ichi Nuclear Power Plant
| Autor: | Hiroyuki Takahashi, Masahide Kubota, Yoshihisa Kubota, Yoshito Watanabe, Satoshi Yoshida, Naoko Shiomi, Yoshiaki Furuhata, Shoichi Fuma, Morihiko Tomozawa, Kazutaka Doi, Shinsuke H. Sakamoto, Fumio Yamada, Isao Kawaguchi, Masanari Aoki, Yusaku Shigemura, Taiki Kawagoshi, Masahiko Mizoguchi |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
biology medicine.diagnostic_test Fukushima Nuclear Accident Ecology Zoology General Chemistry 010501 environmental sciences biology.organism_classification 01 natural sciences 03 medical and health sciences 030104 developmental biology medicine Environmental Chemistry Fish Dose rate 0105 earth and related environmental sciences Apodemus speciosus Fluorescence in situ hybridization |
| Zdroj: | Environ. Sci. Technol.. 51(8):4632-4641 |
| Popis: | Since the Fukushima Dai-ichi Nuclear Power Plant accident, radiation effects on nonhuman biota in the contaminated areas have been a major concern. Here, we analyzed the frequencies of chromosomal aberrations (translocations and dicentrics) in the splenic lymphocytes of large Japanese field mice (Apodemus speciosus) inhabiting Fukushima Prefecture. A. speciosus chromosomes 1, 2, and 5 were flow-sorted in order to develop A. speciosus chromosome-specific painting probes, and FISH (fluorescence in situ hybridization) was performed using these painting probes to detect the translocations and dicentrics. The average frequency of the translocations and dicentrics per cell in the heavily contaminated area was significantly higher than the frequencies in the case of the noncontaminated control area and the slightly and moderately contaminated areas, and this aberration frequency in individual mice tended to roughly increase with the estimated dose rates and accumulated doses. In all 4 sampling areas, the proportion of aberrations occurring in chromosome 2 was approximately >3 times higher than that in chromosomes 1 and 5, which suggests that A. speciosus chromosome 2 harbors a fragile site that is highly sensitive to chromosome breaks induced by cellular stress such as DNA replication. The elevated frequency of chromosomal aberrations in A. speciosus potentially resulting from the presence of a fragile site in chromosome 2 might make it challenging to observe the mild effect of chronic low-dose-rate irradiation on the induction of chromosomal aberrations in A. speciosus inhabiting the contaminated areas of Fukushima. |
| Databáze: | OpenAIRE |
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