Kiloniella antarctica sp. nov., isolated from a polynya of Amundsen Sea in Western Antarctic Sea
| Autor: | Sun-Bin Choi, Hye-Young Yang, Song-Gun Kim, Jong-Geol Kim, Chung Yeon Hwang, Seong Woon Roh, So-Jeong Kim, Sung-Keun Rhee, Man-Young Jung, Ok-Ja Si |
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| Rok vydání: | 2017 |
| Předmět: |
DNA
Bacterial 0301 basic medicine Kiloniella laminariae Antarctic Regions Biology Microbiology Kiloniella 03 medical and health sciences Kiloniella spongiae RNA Ribosomal 16S Botany Seawater Phylogeny Ecology Evolution Behavior and Systematics Alphaproteobacteria Base Composition Phylogenetic tree Strain (chemistry) Fatty Acids Nucleic Acid Hybridization Sequence Analysis DNA General Medicine 16S ribosomal RNA C content Bacterial Typing Techniques genomic DNA 030104 developmental biology |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 67:2397-2402 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijsem.0.001968 |
| Popis: | A taxonomic study was conducted on strain soj2014T, which was isolated from the surface water of a polynya in the Antarctic Sea. Comparative 16S rRNA gene sequence analysis showed that strain soj2014T belongs to the family Kiloniellaceae and is closely related to Kiloniella spongiae MEBiC09566T, ‘ Kiloniella litopenaei ’ P1-1T and Kiloniella laminariae LD81T (98.0 %, 97.8 % and 96.2 % 16S rRNA gene sequence similarity, respectively). The DNA–DNA hybridization values between strain soj2014T and closely related strains were below 28.6 %. The G+C content of the genomic DNA of strain soj2014T was 45.5 mol%. The predominant cellular fatty acids were summed feature 8 (composed of C18 : 1ω6c/C18 : 1ω7c, 57.0 %) and summed feature 3 (composed of C16 : 1ω6c/C16 : 1ω7c, 23.5 %). Strain soj2014T was Gram-stain-negative, slightly curved, spiral-shaped, and motile with a single polar flagellum. The strain grew at 0–30 °C (optimum, 25 °C), in 1.5–5.1 % (w/v) NaCl (optimum, 2.1–2.4 %) and at pH 5.5–9.5 (optimum, 7.5–8.0). It also had differential carbohydrate utilization traits and enzyme activities compared with closely related strains. Based on these phylogenetic, phenotypic and chemotaxonomic analyses, strain soj2014T represents a distinct species, separable from the reference strains, and is, therefore, proposed as a novel species, Kiloniella antarctica sp. nov. The type strain is soj2014T (=KCTC 42186T=JCM 30386T). |
| Databáze: | OpenAIRE |
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