Two-step tumor-targeting therapy via integrating metabolic lipid-engineering with in situ click chemistry
| Autor: | Jinfeng Zhang, Liping Zuo, Wanru Zhuang, Houshun Zhu, Hai-Yan Xie, Guihong Lu, Wei Wei |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Indocyanine Green
Cell Biomedical Engineering Choline Metabolic engineering Mice chemistry.chemical_compound Drug Delivery Systems In vivo Cell Line Tumor Neoplasms Phosphatidylcholine medicine Animals Humans General Materials Science Coloring Agents Tumor microenvironment Chemistry Erythrocyte Membrane Phototherapy Photothermal therapy Lipids Cell biology medicine.anatomical_structure Metabolic Engineering Drug delivery Click chemistry Click Chemistry |
| Zdroj: | Biomaterials Science. 8:2283-2288 |
| ISSN: | 2047-4849 2047-4830 |
| DOI: | 10.1039/d0bm00088d |
| Popis: | Highly efficient tumor-targeted therapy remains a great challenge due to the complexity and heterogeneity of tumor tissues. Herein, we developed an in vivo two-step tumor-targeting strategy by combining metabolic lipid-engineering with a stain-promoted azide-alkyne 1,3-dipolar cycloaddition (SPAAC) reaction, independent of the tumor microenvironment and cell phenotype. Firstly, exogenously-supplied azidoethyl-cholines (AECho) were metabolically incorporated into the cell membranes in tumor tissues through the intrinsic biosynthesis of phosphatidylcholine. The pre-inserted and accumulated azido groups (N3) could subsequently serve as 'artificial chemical receptors' for the specific anchoring of dibenzocyclooctyne (DBCO) modified biomimetic nanoparticles (DBCO-RBCG@ICG) via in situ click chemistry, resulting in significantly enhanced tumor-targeting and then an improved photothermal therapy effect. Such a two-step targeting strategy based on these cutting-edge techniques provided new insights into the universal and precise functionalization of living tissues for site-specific drug delivery in the diagnosis and treatment of various diseases. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|