Cecal microbiota of Tibetan Chickens from five geographic regions were determined by 16S rRNA sequencing
| Autor: | Xin Yang, Shuai Men, Xueyan Zhou, Chang-Wen Xu, Bing-cun Ma, Chaowu Yang, Hongning Wang, Peng Zhang, Chang-Wei Lei, Rong Xiang, Xiaosong Jiang, Qi Xiong, Anyun Zhang |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
China Tibetan Chicken animal structures Daheng broiler chickens Firmicutes 030106 microbiology Tibet Polymerase Chain Reaction digestive system Microbiology 03 medical and health sciences RNA Ribosomal 16S Animals Lohmann egg‐laying hens DGGE Cecum Original Research Bacteria Base Sequence Geography biology Denaturing Gradient Gel Electrophoresis Sequence Analysis RNA Spirochaeta high‐throughput sequencing High-Throughput Nucleotide Sequencing Bacteroidetes biology.organism_classification 16S ribosomal RNA Gastrointestinal Microbiome 030104 developmental biology Cecal microbiota Bacteroides Proteobacteria Chickens Temperature gradient gel electrophoresis Megamonas |
| Zdroj: | MicrobiologyOpen |
| ISSN: | 2045-8827 |
| DOI: | 10.1002/mbo3.367 |
| Popis: | Tibetan Chickens should have unique gastrointestinal microbiota because of their particular habitats. Thus, the aim of this study was to investigate the cecal microbiota of Tibetan Chickens from five typical high‐altitude regions of China. Lohmann egg‐laying hens (LMs) and Daheng broiler chickens (DHs) were chosen as controls. The cecal bacterial populations of Tibetan Chickens were surveyed by high‐throughput sequencing (HTS) of the bacterial 16S rRNA hypervariable region V3‐V4 (16S rRNAV3‐V4) combined with community‐fingerprinting analysis of the 16S rRNA gene based on polymerase chain reaction‐denaturing gradient gel electrophoresis (PCR‐DGGE). The results revealed that the majority of cecal microbiota differed between the Tibetan Chicken and LM/DH. The microbial communities in the cecum were composed of 16 phyla, 28 classes, 36 orders, 57 families, 101 genera, and 189 species. Represented phyla were Bacteroidetes (>47%), Firmicutes (>18.8%), Spirochaetae (>0.3%), and Proteobacteria (>0.4%). Bacteroides and the RC9 gut group were the two most abundant genera. There were relatively more Christensenellaceae, Subdoligranulum, Spirochaeta, and Treponema in Tibetan Chickens, whereas there were more Phascolarctobacterium, Faecalibacterium, Megamonas, and Desulfovibrio in LMs and DHs. The cecal microbiota of Tibetan Chicken have slightly diverged due to exposure to different geographic environments. Differences in the intestinal bacterial communities of Tibetan Chicken and LM/DH were noted. |
| Databáze: | OpenAIRE |
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