Quantitation of cytomegalovirus in the blood of liver transplant recipients
| Autor: | Per Stalhandske, Helena Kilgariff, Elwyn Elias, Anna Matyi-toth, M.D. David Mutimer, James Neuberger, J Shaw, Katharina O'Donnell, Paul McMaster, Bridget Gunson |
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| Rok vydání: | 1995 |
| Předmět: |
Adult
Graft Rejection Male medicine.medical_treatment Congenital cytomegalovirus infection Cytomegalovirus Enzyme-Linked Immunosorbent Assay Liver transplantation Antibodies Viral Antiviral Agents Polymerase Chain Reaction Asymptomatic Predictive Value of Tests medicine Humans Viremia Antiviral treatment CMV immunoglobulin Retrospective Studies Hepatology business.industry Liver Diseases Antiviral therapy virus diseases Middle Aged medicine.disease Dna amplification Virology Liver Transplantation Immunoglobulin M Cytomegalovirus Infections DNA Viral Immunology Drug Therapy Combination Female Surgery medicine.symptom business Viral load Immunosuppressive Agents |
| Zdroj: | Liver Transplantation and Surgery. 1:395-400 |
| ISSN: | 1074-3022 |
| DOI: | 10.1002/lt.500010611 |
| Popis: | An assay for quantitation of cytomegalovirus (CMV) has been developed. The assay combines DNA amplification and enzyme-linked immunosorbent assay (ELISA) detection. In this study, the assay has been used to examine sequential buffy-coats from 32 consecutive liver transplant recipients. In a febrile patient, CMV titres in excess of 10(4) copies per 150,000 cells strongly suggest a diagnosis of symptomatic CMV infection. Antiviral therapy causes a rapid decline in viral titre. Viral titres are seen to rise presymptomatically in some patients. Median peak viral titres differ significantly between symptomatic patients (1.1 x 10(5)), asymptomatic CMV IgM-positive patients (1.7 x 10(3)), and asymptomatic CMV immunoglobulin (Ig)M-negative patients (2.9 x 10(2)). CMV quantitation can be used for diagnosis and surveillance and can also be used to monitor antiviral treatment. |
| Databáze: | OpenAIRE |
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