Filamentous marine fungi as producers of O-glycosylhydrolases: beta-1,3-glucanase from Chaetomium indicum
| Autor: | M. V. Pivkin, Yu. V. Burtseva, N. S. Verigina, V. V. Sova, Tatyana N. Zvyagintseva |
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| Rok vydání: | 2002 |
| Předmět: |
chemistry.chemical_classification
Pacific Ocean biology Geography Size-exclusion chromatography Temperature Ultrafiltration Cellulase Glucan 1 3-beta-Glucosidase Glucanase Chaetomium Hydrogen-Ion Concentration Chromatography Ion Exchange Applied Microbiology and Biotechnology Substrate Specificity chemistry.chemical_compound Enzyme Biosynthesis chemistry Biochemistry biology.protein Extracellular Chromatography Gel Amylase Marine fungi |
| Zdroj: | Marine biotechnology (New York, N.Y.). 5(4) |
| ISSN: | 1436-2228 |
| Popis: | Ninety fungal strains (42 species) isolated from marine habitats were studied for their ability to produce extracellular enzymes. Cultural filtrates of these strains were shown to contain a series of glycosidases (beta-glucosidases, N-acetyl-beta-glucosaminidases, beta-galactosidases alpha-mannosidases) and glucanases (1,3-beta-glucanases, amylases) which varied with habitat. The level of activity depended on the species of fungi. Several promising strains capable of producing both individual enzymes and a set of enzymes for splitting carbohydrate-containing compound have been isolated. Optimal conditions for growth of Chaetomium indicum and for biosynthesis of beta-1,3-glucanase were determined. beta-1,3-Glucanase was isolated using ion-exchange chromatography, ultrafiltration, and gel filtration. The presence of 2 enzyme forms was shown; both forms were exo-beta-1,3-glucanases. |
| Databáze: | OpenAIRE |
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