In Vitro Biological Activities of a Series of 2.BETA.-Substituted Analogues of 1.ALPHA.,25-Dihydroxyvitamin D3
| Autor: | Keiichi Ozono, Mayuko Kurobe, Toshio Okano, Yoshiyuki Ono, Naoko Tsugawa, Kimie Nakagawa, Noboru Kubodera |
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| Rok vydání: | 2000 |
| Předmět: |
Transcriptional Activation
Stereochemistry Pharmaceutical Science HL-60 Cells Transfection Binding Competitive Calcitriol receptor HeLa Structure-Activity Relationship Transactivation Cytochrome P-450 Enzyme System Animals Humans Luciferase Vitamin D Vitamin D3 24-Hydroxylase Cholecalciferol Pharmacology Reporter gene biology Vitamin D-Binding Protein Biological activity General Medicine biology.organism_classification In vitro Rats Kinetics Biochemistry Steroid Hydroxylases Receptors Calcitriol Cattle Cell Division Protein Binding |
| Zdroj: | Biological and Pharmaceutical Bulletin. 23:66-71 |
| ISSN: | 1347-5215 0918-6158 |
| DOI: | 10.1248/bpb.23.66 |
| Popis: | Biological activities of a series of 2beta-substituted analogues of 1alpha,25-dihydroxyvitamin D3 [1alpha,25(OH)2D3] were evaluated in vitro in terms of their binding affinity with regard to calf thymus cytosolic vitamin D receptor (VDR) and rat plasma vitamin D-binding protein (DBP). Additionally, reporter gene luciferase activities using either a rat 25-hydroxyvitamin D3-24-hydroxylase gene promoter, including two vitamin D-responsive elements (VDREs), in transfected rat osteoblast-like ROS17/2.8 cells, or a human VDR-GAL4 modified two-hybrid system in transfected human epitheloid carcinoma, cervix HeLa cells were examined. Binding affinity for VDR, transactivation potency on the target gene and VDR-mediated gene regulation of the hydroxyalkyl and hydroxyalkoxy 2beta-substituted analogues were almost comparable to those of 1alpha,25(OH)2D3, while the alkyl and alkenyl analogues were much less active than 1alpha,25(OH)2D3. This study investigated the biological evaluation of a series of 2beta-substituted analogues at the molecular level, with regard to the structural differences of alkyl, alkenyl, hydroxyalkyl, hydroxyalkoxy, alkoxy, hydroxy and chloro substituents at the 2beta-position of 1alpha,25(OH)2D3. |
| Databáze: | OpenAIRE |
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