Indocyanine green loaded hyaluronan-derived nanoparticles for fluorescence-enhanced surgical imaging of pancreatic cancer

Autor: Joshua J. Souchek, Samuel M. Cohen, Michael A. Hollingsworth, Nicholas E. Wojtynek, Bowen Qi, Aaron M. Mohs, Quan P. Ly, Megan B. Holmes, Graça Almeida-Porada, Ayrianne J. Crawford
Rok vydání: 2018
Předmět:
Indocyanine Green
Pathology
medicine.medical_specialty
Biomedical Engineering
Pharmaceutical Science
Medicine (miscellaneous)
Bioengineering
02 engineering and technology
Article
Fluorescence
Fluorescence image-guided surgery
Mice
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Phagocytosis
In vivo
Pancreatic cancer
Hyaluronic acid
Tumor Cells
Cultured
medicine
Fluorescence microscope
Animals
General Materials Science
Hyaluronic Acid
Chemistry
Chemotaxis
Optical Imaging
021001 nanoscience & nanotechnology
medicine.disease
Mice
Inbred C57BL
Pancreatic Neoplasms
Disease Models
Animal
medicine.anatomical_structure
Microscopy
Fluorescence
Surgery
Computer-Assisted
030220 oncology & carcinogenesis
Toxicity
Nanoparticles
Molecular Medicine
Female
0210 nano-technology
Pancreas
Indocyanine green
Carcinoma
Pancreatic Ductal
Zdroj: Nanomedicine: Nanotechnology, Biology and Medicine. 14:769-780
ISSN: 1549-9634
Popis: Pancreatic ductal adenocarcinoma is highly lethal and surgical resection is the only potential curative treatment for the disease. In this study, hyaluronic acid derived nanoparticles with physico-chemically entrapped indocyanine green, termed NanoICG, were utilized for intraoperative near infrared fluorescence detection of pancreatic cancer. NanoICG was not cytotoxic to healthy pancreatic epithelial cells and did not induce chemotaxis or phagocytosis, it accumulated significantly within the pancreas in an orthotopic pancreatic ductal adenocarcinoma model, and demonstrated contrast-enhancement for pancreatic lesions relative to non-diseased portions of the pancreas. Fluorescence microscopy showed higher fluorescence intensity in pancreatic lesions and splenic metastases due to NanoICG compared to ICG alone. The in vivo safety profile of NanoICG, including, biochemical, hematological, and pathological analysis of NanoICG-treated healthy mice, indicates negligible toxicity. These results suggest that NanoICG is a promising contrast agent for intraoperative detection of pancreatic tumors.
Databáze: OpenAIRE
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