Surface topography as a tool to detect early changes in a posttraumatic equine model of osteoarthritis
Autor: | Parvathy Thampi, Michel P. Laurent, Suzanne M Tabbaa, Marcus A Wimmer, David D. Frisbie, C. Wayne McIlwraith, Brian Johnstone |
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Rok vydání: | 2021 |
Předmět: |
Cartilage
Articular musculoskeletal diseases Orthodontics Carpal Joint Carpal Joints Radial carpal bone business.industry Cartilage H&E stain Articular cartilage Osteoarthritis medicine.disease Cartilage surface medicine.anatomical_structure Third carpal bone Animals Medicine Horse Diseases Orthopedics and Sports Medicine Horses business Cartilage Diseases |
Zdroj: | Journal of Orthopaedic Research. 40:1349-1357 |
ISSN: | 1554-527X 0736-0266 |
DOI: | 10.1002/jor.25175 |
Popis: | The equine model of posttraumatic osteoarthritis (OA) mimics certain aspects of the naturally occurring disease, both in horses and humans. The objective of this study was to assess articular cartilage degeneration in a posttraumatic OA model using the established macroscopic and microscopic scoring systems and compare them with a novel surface topography analysis. OA was induced in the carpal joint of 15 (n = 15) mixed breed horses. Surface changes on the articular cartilage were characterized using osteochondral blocks from the third carpal bone (C3) and radial carpal bone using surface topography, standard histological grading, and gross evaluation of the joints. Significant differences were observed between OA and non-OA joints for gross evaluation scores. Microscopic scores of hematoxylin and eosin and Safranin O and Fast Green-stained sections demonstrated no differences between OA and non-OA joints. However, articular cartilage from the induced OA joint had significantly greater surface topography measurements compared with the sham treatment group, consistent with the changes seen on gross evaluation of joints. No significant correlations were noted between surface roughness measurements, histological assessment, and gross evaluation scores. The results suggest that surface topography analysis may provide a reliable objective approach to assess early changes in the cartilage surface in OA. |
Databáze: | OpenAIRE |
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