Overexpression miR-211-5p hinders the proliferation, migration, and invasion of thyroid tumor cells by downregulating SOX11
| Autor: | Zhi-min Shi, Xiao-juan Shang, Feng Xi, Lei Wang, Dong-ling Jin, Yan-feng Shen |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Clinical Biochemistry Thyroid Gland Down-Regulation Mice Nude Biology SOXC Transcription Factors Flow cytometry Mice 03 medical and health sciences 0302 clinical medicine Cell Movement In vivo microRNA medicine Animals Humans Immunology and Allergy Neoplasm Invasiveness MTT assay Thyroid Neoplasms Research Articles Cell Proliferation medicine.diagnostic_test Cell growth Biochemistry (medical) Public Health Environmental and Occupational Health Reproducibility of Results Hematology Cell cycle Molecular biology MicroRNAs Medical Laboratory Technology 030104 developmental biology Apoptosis Cell culture 030220 oncology & carcinogenesis |
| Zdroj: | Journal of Clinical Laboratory Analysis. 32:e22293 |
| ISSN: | 0887-8013 |
| DOI: | 10.1002/jcla.22293 |
| Popis: | Purpose This study was aimed to investigate the relationship between miR-211-5p and SOX11, and the effects of their interaction on the proliferation, viability, and invasion of human thyroid cancer (TC) cells. Methods We used quantitative real-time PCR (qRT-PCR) to determine the expression of miR-211-5p and SOX11mRNA in the thyroid tumorous and the adjacent tissues. The target relationship between miR-211-5p and SOX11 was confirmed using dual luciferase reporter gene assay. Flow cytometry, colony formation assay, Transwell assay, and MTT assay were performed to determine the cell-cycle progression, cell apoptosis, proliferation and invasion, respectively. In addition, the tumor formation assay in nude mice was done to assess the effect of miR-211-5p on TC development in vivo. Results MiR-211-5p was underexpressed, whereas SOX11 was overexpressed in TC. The overexpression of miR-211-5p inhibited the expression of SOX11. The cell cycle was arrested and the proliferation as well as invasiveness was suppressed by exogenous miR-211-5p in TC cell line. The antitumor role of miR-211-5p was proved by the animal experiment. Conclusion MiR-211-5p affected the viability, proliferation and invasion of TC by negatively regulating SOX11 expression. |
| Databáze: | OpenAIRE |
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