Differential stimulation of the retina with glutamate toward a neurotransmitter-based retinal prosthesis
| Autor: | John B. Troy, Samsoon Inayat, Laxman Saggere, Corey M. Rountree |
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| Rok vydání: | 2016 |
| Předmět: |
0303 health sciences
Retina genetic structures Glutamate receptor Retinal Stimulation Anatomy Biology Retinal ganglion eye diseases 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine.anatomical_structure chemistry Inner nuclear layer medicine Excitatory postsynaptic potential sense organs Neurotransmitter Neuroscience 030217 neurology & neurosurgery 030304 developmental biology |
| DOI: | 10.1101/060681 |
| Popis: | Subretinal stimulation of the retina with neurotransmitters, the normal means of conveying visual information, is a potentially better alternative to electrical stimulation widely used in current retinal prostheses for treating blindness from photoreceptor degenerative diseases. Yet, no retinal stimulation study exploiting the inner retinal pathways exists. Here, we demonstrate the feasibility of differentially stimulating retinal ganglion cells (RGCs) through the inner nuclear layer of the retina with glutamate, a primary neurotransmitter chemical, in a biomimetic way. We show that controlled pulsatile delivery of glutamate into the subsurface of explanted wild-type rat retinas elicits highly localized simultaneous inhibitory and excitatory spike rate responses in OFF and ON RGCs. We also present the spatiotemporal characteristics of RGC responses to subretinally injected glutamate and the therapeutic stimulation parameters. Our findings could pave the way for future development of a neurotransmitter-based subretinal prosthesis offering more naturalistic vision and better visual acuity than electrical prostheses. |
| Databáze: | OpenAIRE |
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