S100A7 in Psoriasis: Immunodetection and Activation by CRISPR technology
| Autor: | Fabio D’Amico, Evangelia Skarmoutsou, Maria Clorinda Mazzarino, Mariagrazia Granata |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Keratinocytes
Transcriptional Activation 0301 basic medicine S100A7 Immunofluorescence Fluorescent Antibody Technique EF-hand S100 Calcium Binding Protein A7 Cell Line Pathogenesis 03 medical and health sciences 0302 clinical medicine Immune system Downregulation and upregulation Psoriasis Humans Medicine CRISPR Calcium Immunohistochemistry COP9 Signalosome Complex CRISPR-Cas Systems Intracellular Signaling Peptides and Proteins Peptide Hydrolases Up-Regulation medicine.diagnostic_test business.industry medicine.disease 030104 developmental biology Immunology business 030215 immunology |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493990290 |
| DOI: | 10.1007/978-1-4939-9030-6_45 |
| Popis: | Psoriasis, an inflammatory autoimmune skin disease, is the result of a chronic interaction between hyperproliferative keratinocytes and infiltrating activated immune cells. The mechanisms underlying psoriasis pathogenesis remain largely unknown, although a combination of genetic and environmental factors plays an important role in its development. S100A7 is overexpressed in psoriasis, and there is growing evidence that S100A7 may be involved in the pathogenesis of psoriasis. Since the mechanisms underlying S100A7 regulation and function remain elusive, a better understanding of these mechanisms may be useful to uncover additional treatment approaches for psoriasis. Immunohistology provides invaluable tools for a better understanding of the pathogenetic mechanisms of psoriasis. Here, we describe basic methods for immunofluorescence and immunohistochemistry analysis of S100A7 expression in psoriatic patients as well as in S100A7 CRISPR-activated human keratinocyte cell line. |
| Databáze: | OpenAIRE |
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