Involvement of rho kinase in the ouabain-induced contractions of the rat renal arteries
| Autor: | Huseyin Beydagi, Emel Songu-Mize, Mustafa Ark, Havva Kubat, Tolgay Ergenoglu |
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| Rok vydání: | 2006 |
| Předmět: |
Male
medicine.medical_specialty Vascular smooth muscle Pyridines Biophysics macromolecular substances Protein Serine-Threonine Kinases Biochemistry Muscle Smooth Vascular Ouabain Myosin-Light-Chain Phosphatase chemistry.chemical_compound Renal Artery Internal medicine Myosin medicine Animals Enzyme Inhibitors Phosphorylation Rats Wistar Molecular Biology Rho-associated protein kinase rho-Associated Kinases Intracellular Signaling Peptides and Proteins Fasudil Cell Biology Amides Rats Isoenzymes Y-27632 Endocrinology chemistry Vasoconstriction Myosin binding medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 340:417-421 |
| ISSN: | 0006-291X |
| Popis: | Agonist and depolarization-induced vascular smooth muscle contractions include the activation of rho/rho kinase pathway. However, there are no reports addressing the question whether this pathway is involved in ouabain-induced vascular smooth muscle contractions. Therefore, in this study, the possible participation of the rho/rho kinase pathway in ouabain-induced contractions was evaluated in rat renal arteries. Effects of rho kinase inhibitors (fasudil and Y-27632) on ouabain-induced contractions, and phosphorylation of myosin binding subunits (MYPT/MBS85) of myosin phosphatase were determined using isolated tissue and Western blot experiments, respectively. Fasudil and Y-27632 inhibited ouabain-induced contractions in a concentration-dependent manner. The phosphorylation of MYPT was not altered by ouabain. However, ouabain significantly increased MBS85 phosphorylation of myosin phosphatase. The phosphorylation of both subunits of myosin phosphatase was inhibited by Y-27632. These results indicate that activation of rho kinase and the subsequent phosphorylation of MBS85 are involved in ouabain-induced contraction of rat renal arteries. This mechanism may be important in essential hypertension with elevated endogenous ouabain levels. (c) 2005 Elsevier Inc. All rights reserved. |
| Databáze: | OpenAIRE |
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