Trophectoderm lineage determination in cattle
| Autor: | Martyn Donnison, David N. Wells, Craig S. Smith, Peter L. Pfeffer, David J. Pearton, D.K. Berg, Ric Broadhurst |
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| Rok vydání: | 2010 |
| Předmět: |
Transcription
Genetic Molecular Sequence Data Plasma protein binding Biology General Biochemistry Genetics and Molecular Biology Mice Species Specificity Transcription (biology) Genes Reporter Ectoderm Inner cell mass Animals Cell Lineage Binding site CDX2 Molecular Biology Gene reproductive and urinary physiology Regulation of gene expression Homeodomain Proteins Base Sequence Gene Expression Regulation Developmental Cell Biology Cell biology Trophoblasts Enhancer Elements Genetic Blastocyst Inner Cell Mass Gene Knockdown Techniques Immunology embryonic structures Cattle Rabbits Stem cell biology Octamer Transcription Factor-3 Developmental Biology Protein Binding |
| Zdroj: | Developmental cell. 20(2) |
| ISSN: | 1878-1551 |
| Popis: | SummaryThe trophectoderm (TE) and inner cell mass (ICM) are committed and marked by reciprocal expression of Cdx2 and Oct4 in mouse late blastocysts. We find that the TE is not committed at equivalent stages in cattle, and that bovine Cdx2 is required later, for TE maintenance, but does not repress Oct4 expression. A mouse Oct4 (mOct4) reporter, repressed in mouse TE, remained active in the cattle TE; bovine Oct4 constructs were not repressed in the mouse TE. mOct4 has acquired Tcfap2 binding sites mediating Cdx2-independent repression—cattle, humans, and rabbits do not contain these sites and maintain high Oct4 levels in the TE. Our data suggest that the regulatory circuitry determining ICM/TE identity has been rewired in mice, to allow rapid TE differentiation and early blastocyst implantation. These findings thus emphasize ways in which mice may not be representative of the earliest stages of mammalian development and stem cell biology. |
| Databáze: | OpenAIRE |
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