Thrombin generation assays are versatile tools in blood coagulation analysis: A review of technical features, and applications from research to laboratory routine
| Autor: | Stephan Schwers, Julia Mueller, Peter Turecek, Nikolaus B Binder, Matthias Germer, François Depasse, Björn Hermes, Thomas Dr. Wissel |
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| Rok vydání: | 2021 |
| Předmět: |
medicine.drug_class
business.industry Antithrombin Anticoagulant Thrombin Hematology Computational biology Fibrinogen Thrombin generation Coagulation Hemostasis medicine Coagulation testing Humans Blood Coagulation Tests business Blood Coagulation Laboratories Clinical circulatory and respiratory physiology medicine.drug |
| Zdroj: | Journal of Thrombosis and Haemostasis. 19:2907-2917 |
| ISSN: | 1538-7836 |
| Popis: | Thrombin is the pivotal enzyme in the biochemistry of secondary hemostasis crucial to maintaining homeostasis of hemostasis. In contrast to routine coagulation tests (PT or aPTT) or procoagulant or anticoagulant factor assays (e.g. fibrinogen, factor VIII, antithrombin or protein C), the thrombin generation assay (TGA), also named thrombin generation test (TGT) is a so-called "global assay" that provides a picture of the hemostasis balance though a continuous and simultaneous measurement of thrombin formation and inhibition. First described in the early 1950s, as a manual assay, efforts have been made in order to standardize and automate the assay to offer researchers, clinical laboratories and the pharmaceutical industry a versatile tool covering a wide range of clinical and non-clinical applications. This review describes technical options offered to properly run TGA, including a review of preanalytical and analytical items, performance, interpretation, and applications in physiology research and pharmacy. |
| Databáze: | OpenAIRE |
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