Extensive Genetic Diversity of HIV-1 in Incident and Prevalent Infections among Malaysian Blood Donors: Multiple Introductions of HIV-1 Genotypes from Highly Prevalent Countries

Autor: Kok Keng Tee, Fread Anderios, Michael P. Busch, Hazwan Abdul Halim, Abdul Hamid Bon, Adeeba Kamarulzaman, Wei Zhen Chow, Sheila M. Keating, Yutaka Takebe
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
RNA viruses
Male
Physiology
Population genetics
lcsh:Medicine
Blood Donors
HIV Infections
Pathology and Laboratory Medicine
Biochemistry
gag Gene Products
Human Immunodeficiency Virus
Geographical Locations
Immunodeficiency Viruses
Genotype
Blood plasma
Medicine and Health Sciences
Prevalence
Clade
lcsh:Science
Phylogeny
Data Management
Genetics
Multidisciplinary
Phylogenetic tree
Incidence
virus diseases
Phylogenetic Analysis
Hematology
Body Fluids
Nucleic acids
Phylogenetics
Blood
Medical Microbiology
Viral Pathogens
Viruses
Female
Pathogens
Anatomy
Research Article
Adult
Computer and Information Sciences
Asia
DNA recombination
Biology
Research and Analysis Methods
Microbiology
Blood Plasma
03 medical and health sciences
Retroviruses
Humans
Avidity
Evolutionary Systematics
Molecular Biology Techniques
Microbial Pathogens
Molecular Biology
Taxonomy
Genetic diversity
Molecular Biology Assays and Analysis Techniques
Evolutionary Biology
Population Biology
lcsh:R
Lentivirus
Organisms
Malaysia
Biology and Life Sciences
HIV
Genetic Variation
DNA
Sequence Analysis
DNA
030112 virology
Virology
Health Care
030104 developmental biology
pol Gene Products
Human Immunodeficiency Virus
People and Places
HIV-1
lcsh:Q
Population Genetics
Zdroj: PLoS ONE
PLoS ONE, Vol 11, Iss 8, p e0161853 (2016)
ISSN: 1932-6203
Popis: Transfusion-transmissible infections including HIV-1 continue to pose major risks for unsafe blood transfusions due to both window phase infections and divergent viruses that may not be detected by donor screening assays. Given the recent emergence of several HIV-1 circulating recombinant forms (CRFs) in high-risk populations in the Southeast Asia region, we investigated the genetic diversity of HIV-1 among the blood donors in Kuala Lumpur, Malaysia. A total of 211 HIV-positive plasma samples detected among 730,188 donations to the National Blood Centre between 2013 and 2014 were provided (90.5% male, median age: 27.0 years old). Recent or long-term infection status at the time of donation was determined using a limiting antigen avidity enzyme immunoassay (LAg-Avidity EIA). HIV-1 gag-pol genes were amplified and sequenced from residual plasma for 149 cases followed by genotype determination using phylogenetic and recombination analyses. Transmitted antiretroviral resistance mutations were not observed among the blood donors, among which 22.7% were classified as recent or incident infections. Major circulating HIV-1 genotypes determined by neighbour-joining phylogenetic inference included CRF01_AE at 40.9% (61/149), CRF33_01B at 21.5% (32/149), and subtype B at 10.1% (15/149). Newly-described CRFs including CRF54_01B circulated at 4.0%, CRF74_01B at 2.0%, and CRF53_01B and CRF48_01B at 0.7% each. Interestingly, unique HIV-1 genotypes including African subtype G (8.7%), CRF45_cpx (1.3%), CRF02_AG (0.7%) and CRF07_BC (0.7%) from China were detected for the first time in the country. A cluster of subtype G sequences formed a distinct founder sub-lineage within the African strains. In addition, 8.7% (13/149) of HIV-infected donors had unique recombinant forms (URFs) including CRF01_AE/B' (4.7%), B'/C (2.7%) and B'/G (1.3%) recombinants. Detailed analysis identified similar recombinant structures with shared parental strains among the B'/C and B'/G URFs, some of which were sequenced from recently infected individuals, indicating the possible emergence and on-going spread of foreign clades of CRF candidates among the local population. The findings demonstrate extensive molecular complexity of HIV-1 among the infected blood donors in Malaysia, driven in part by the increased spread of recently described CRFs and multiple introductions of previously unreported genotypes from highly prevalent countries.
Databáze: OpenAIRE
Externí odkaz: