Enzymatic hydrolysis of ammonia-treated rice straw
| Autor: | Bruce E. Dale, Betzabé Sulbarán-de-Ferrer, Graciela Ojeda-de-Rodriguez, Marielena Aristiguieta, Alexis Ferrer |
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| Rok vydání: | 2003 |
| Předmět: |
Arabinose
Bioengineering Cellulase Xylose Applied Microbiology and Biotechnology Biochemistry chemistry.chemical_compound Hydrolysis Bioreactors Ammonia Polysaccharides Enzymatic hydrolysis Monosaccharide Molecular Biology Chromatography High Pressure Liquid chemistry.chemical_classification Chromatography biology Chemistry Monosaccharides food and beverages Galactose Fructose Agriculture Oryza General Medicine Enzymes Glucose Anhydrous biology.protein Indicators and Reagents Biotechnology |
| Zdroj: | Applied biochemistry and biotechnology. |
| ISSN: | 0273-2289 |
| Popis: | Rice straw pretreated with liquid anhydrous ammonia was hydrolyzed with cellulase, cellobiase, and hemicellulase. Ammonia-processing conditions were 1.5 g of NH3/g of dry matter, 85 degrees C, and several sample moisture contents. There were four ammonia addition time (min)-processing time (min) combinations. Sugars produced were analyzed as reducing sugars (dinitrosalicylic acid method) and by high-performance liquid chromatography. Monomeric sugars increased from 11% in the nontreated rice straw to 61% of theoretical in treated rice straw (79.2% conversion as reducing sugars). Production of monosaccharides was greater at higher moisture content and was processing time dependent. Glucose was the monosaccharide produced in greater amounts, 56.0%, followed by xylose, arabinose, and fructose, with 35.8, 6.6, and 1.4%, respectively. |
| Databáze: | OpenAIRE |
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