Thrombogenicity and procoagulant activity of human mesothelial cells
| Autor: | J. J. Sixma, T J van Vroonhoven, A. A. G. M. Hoynck Van Papendrecht, A. Pronk, P. G. De Groot, Piet Leguit, Henri A. Verbrugh |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Fibrin
Platelet Aggregation Heparin Thrombogenicity Thrombosis Biology Molecular biology Blood Coagulation Factors Epithelium Thromboplastin Mesothelium Extracellular matrix Tissue factor medicine.anatomical_structure Cell culture Immunology medicine biology.protein Humans Collagen Cardiology and Cardiovascular Medicine Cells Cultured Ex vivo Mesothelial Cell |
| Zdroj: | Arteriosclerosis and Thrombosis: A Journal of Vascular Biology. 12:1428-1436 |
| ISSN: | 1049-8834 |
| DOI: | 10.1161/01.atv.12.12.1428 |
| Popis: | Cell seeding may decrease the thrombogenicity of implanted vascular grafts, but its application is hampered by the limited availability of autologous endothelial cells. We studied the interaction of alternate cells, human peritoneal mesothelial cells, with whole blood in a flow chamber. When citrated blood was perfused over mesothelial cells, platelet adhesion was seen on the intercellular matrix but not on the cells themselves. Perfusions with blood anticoagulated with low-molecular-weight heparin resulted in fibrin formation at the surface of mesothelial cells but not at the surface of human umbilical venous endothelial cells. At shear rates of 200 sec-1 fibrin deposition on the mesothelial cell surface increased during the first 5 minutes to 5.7 +/- 1.06 micrograms fibrin per square centimeter, whereafter these values stabilized. The procoagulant activity of cultured mesothelial cells was higher than that of peritoneal membrane studied ex vivo. However, cultured mesothelial cells incubated with polyclonal antibodies against tissue factor showed a significant decrease in procoagulant activity. We conclude that human peritoneal mesothelial cells may be used for cell seeding procedures, provided that their tissue factor expression can be controlled. |
| Databáze: | OpenAIRE |
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