Integrated microRNA-mRNA analysis of pancreatic ductal adenocarcinoma
| Autor: | Ren H, Huilai Zhang, Han Yt, Wenhua Jiang, He Lf, Pengfei Liu |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Microarray
Gene regulatory network Biology Bioinformatics Pancreatic cancer microRNA Genetics medicine Humans Gene silencing Gene Regulatory Networks RNA Messenger Molecular Biology Oligonucleotide Array Sequence Analysis Messenger RNA Gene Expression Profiling General Medicine Reference Standards medicine.disease Gene Expression Regulation Neoplastic Pancreatic Neoplasms Gene expression profiling MicroRNAs Cancer research Adenocarcinoma Carcinoma Pancreatic Ductal |
| Zdroj: | Genetics and Molecular Research. 14:10288-10297 |
| ISSN: | 1676-5680 |
| DOI: | 10.4238/2015.august.28.14 |
| Popis: | The main aim of this study was to explore the underlying molecular mechanisms and potential target molecules of pancreatic adenocarcinoma. The miRNA (GSE32678) and mRNA (GSE32676) expression profiles of patients with pancreatic ductal adenocarcinoma and healthy controls were downloaded from the Gene Expression Omnibus database. Differentially expressed miRNA and differentially expressed genes were identified by analyzing the microarray algorithm after data preprocessing. Functional analysis was conducted by the Database for Annotation, Visualization and Integrated Analysis. miRNA-mRNA regulation pairs were obtained in TarMir database. The node degree of hsa-miR-200c, hsa-miR-429, and hsa-miR-200b (miRNA), and EFNB2, MYRIP, and PHF17 (mRNA) were extremely high in the miRNA-mRNA network, indicating that these miRNA and mRNA may play a key role in the development of pancreatic cancer. Our study screened out some target miRNAs and mRNAs for pancreatic ductal adenocarcinoma, which may be helpful in its diagnosis and treatment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|