Partial purification of the organism of psittacosis grown in chicken embryo yolk sac
| Autor: | Warren G. Dorsey, W C Patrick, William N. Shirey |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1968 |
| Předmět: |
Antigenicity
Sucrose General Immunology and Microbiology chemistry.chemical_element Embryo General Medicine Metabolism Chick Embryo Biology Calcium General Biochemistry Genetics and Molecular Biology Microbiology chemistry.chemical_compound medicine.anatomical_structure chemistry Biochemistry medicine Methods Animals Centrifugation General Pharmacology Toxicology and Pharmaceutics Yolk sac Chlamydia Digestion Research Article |
| Popis: | A procedure described for the partial purification of suspensions of the organism of psittacosis utilizes tryptic digestion, calcium chloridedextran sulfate precipation, and centrifugation through a sucrose barrier. More than 50% of the organisms in the original suspension are recovered in 80% of the original volume with approximately 95% of the lipids and 85% of the proteins removed. This method is suggested as a preliminary purification procedure in laboratories desiring relatively large amounts of purified agent for studies on metabolism, physical properties, or antigenicity of the organism. Significant aspects of the investigations leading to the selection of the procedure are included. |
| Databáze: | OpenAIRE |
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