Interphase fluorescence in situ hybridization improves the detection of malignant cells in effusions from breast cancer patients
| Autor: | M Fiegl, Johannes Drach, L Müllauer, M Pecherstorfer, J Angerler, N. Zojer, Heinz Huber, Andrea Gsur, S. Roka |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Adult
Cancer Research medicine.medical_specialty Pathology Pleural effusion Centromere Aneuploidy Breast Neoplasms Biology Malignancy Breast cancer Cytology medicine Chromosomes Human Humans Neoplasm Metastasis Interphase In Situ Hybridization Fluorescence Aged Neoplasm Staging Aged 80 and over medicine.diagnostic_test Chromosomes Human Pair 11 Cytogenetics Ascites Chromosome Mapping medicine.disease Pleural Effusion Oncology Cytopathology Female Chromosomes Human Pair 18 Chromosomes Human Pair 7 Chromosomes Human Pair 17 Research Article Fluorescence in situ hybridization |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| DOI: | 10.1038/bjc.1997.65 |
| Popis: | In diagnostic evaluation of effusions, difficulties are encountered when atypical reactive mesothelial cells have to be differentiated from malignant cells. We tested the impact of fluorescence in situ hybridization (FISH) to identify metastatic cells in breast cancer effusions by detection of numerical chromosomal changes. Pleural and ascitic fluid samples (n=57) from 41 breast cancer patients were concomitantly evaluated by routine cytology and FISH, using centromere-specific probes representing chromosomes 7, 11, 12, 17 and 18. After setting stringent cut-off levels deduced from non-malignant control effusions (n=9), the rates of cells with true aneuploidy were determined in each effusion sample from breast cancer patients. The occurrence of aneuploid cells, as detected by FISH and indicative of malignancy, was correlated with the cytological findings. Routine cytology revealed malignancy in 60% of effusions. Using FISH, aneuploid cell populations could be observed in 94% of cytologically positive and in 48% of cytologically negative effusions, thus reverting diagnosis to malignancy. To confirm malignancy in cases with a low frequency of aneuploid cells, two-colour FISH was additionally performed and indeed showed heterogeneous chromosomal aneuploidy within single nuclei. We conclude that FISH is a valuable tool in the diagnosis of malignancy and may serve as an adjunct to routine cytological examination, as demonstrated here for breast cancer effusions. Images Figure 1 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|