Expression of mRNAs for neuropeptide receptors and beta-adrenergic receptors in human osteoblasts and human osteogenic sarcoma cells
| Autor: | Shigeru Mizutani, Yasuko Koshihara, Michitsugu Arai, Toshiharu Nagatsu, Akifumi Togari, Shigeki Mizutani |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
musculoskeletal diseases
Adult Male Receptors Neuropeptide medicine.medical_specialty Adrenergic receptor Receptor expression Vasoactive intestinal peptide Biology Substance-P Receptor Cell Line Internal medicine Receptors Adrenergic beta medicine Humans RNA Messenger Receptor Autocrine signalling Osteosarcoma Osteoblasts General Neuroscience Osteoblast Neuropeptide Y receptor Cell biology medicine.anatomical_structure Endocrinology hormones hormone substitutes and hormone antagonists |
| Zdroj: | Neuroscience letters. 233(2-3) |
| ISSN: | 0304-3940 |
| Popis: | In human periosteum-derived osteoblastic cells (SaM-1) and human osteosarcoma-derived cells (SaOS-2, HOS, MG-63), the mRNA expressions of calcitonin gene-related peptide receptor (CGRP-R), substance P receptor (SP-R), neuropeptide Y receptor (NPY-R), β -adrenergic receptors ( β 1-R, β 2-R, β 3-R), vasoactive intestinal polypeptide type 1 and type 2 receptors (VIP-1R, VIP-2R) and pituitary adenylate cyclase activating polypeptide receptor (PACAP-R) were examined by reverse transcription-polymerase chain reaction (RT-PCR). According to the magnitude of the mRNA expression of alkaline phosphatase (ALP), the relative state of commitment of these osteoblastic cell lines to the osteoblast lineage was SaM-1>SaOS-2>HOS>MG-63. CGRP-R, NPY-R, VIP-1R and β 2-R, but not SP-R, VIP-2R, PACAP-R, β 1-R and β 3-R, were expressed in osteoblasts as well as osteosarcoma cells. Expression of these receptors seems to be a common feature in osteoblastic cells, but the magnitude of expression was not dependent upon the relative state of commitment of the osteoblastic cells to the osteoblast lineage. In addition, VIP mRNA was not expressed in osteoblastic cells, suggesting the absence of an autocrine system of VIP in osteoblasts. These observations suggest that these neuropeptides and norepinephrine are involved in local regulation of human bone metabolism. |
| Databáze: | OpenAIRE |
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