Development of a simple passive haemagglutination-inhibition assay for Listeria monocytogenes lipoteichoic acid
| Autor: | Hans van Dijk, AD C.J.M. Antonissen, Jan M.N. Willers, Kok P. M. van Kessel |
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| Rok vydání: | 1981 |
| Předmět: |
Lipopolysaccharides
Male Staphylococcus aureus Erythrocytes Immunology Phosphatidic Acids Biology medicine.disease_cause Microbiology Mice chemistry.chemical_compound Rabbit antiserum Haemagglutination inhibition assay Listeria monocytogenes Lactobacillus medicine Animals Immunology and Allergy Sheep Immune Sera Galactose Hemagglutination Tests Hemagglutination Inhibition Tests biology.organism_classification Teichoic Acids Agglutination (biology) chemistry Biochemistry Listeria Rabbits Lipoteichoic acid |
| Zdroj: | Journal of Immunological Methods. 44:351-357 |
| ISSN: | 0022-1759 |
| Popis: | A simple assay for listeria lipoteichoic acid was devised. It is based on the property of this substance to attach spontaneously to erythrocytes. Thus coated rabbit erythrocytes were agglutinated by a rabbit antiserum raised towards dead listeria. Inhibition of this passive agglutination was used as a measure of listeria lipoteichoic acid. Cross-reactivity of lipoteichoic acid from Lactobacillus fermenti in the assay and inhibition by simple sugars suggest that the assay is specific for terminal galactose. |
| Databáze: | OpenAIRE |
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