Variable within- and between-Herd Diversity of CTX-M Cephalosporinase-Bearing Escherichia coli Isolates from Dairy Cattle
| Autor: | Matthew F. Weeman, Wondwossen A. Gebreyes, Joshua B. Daniels, Thomas E. Wittum, Dixie F. Mollenkopf, Jennifer L. Mathews, Melanie Abley |
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| Rok vydání: | 2012 |
| Předmět: |
Genotype
Public Health Microbiology Biology medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Feces Plasmid Salmonella Escherichia coli medicine Pulsed-field gel electrophoresis Animals Cluster Analysis Cephalosporinase Ohio Ecology Genetic Variation biochemical phenomena metabolism and nutrition bacterial infections and mycoses DNA Fingerprinting Drug Utilization Anti-Bacterial Agents Bacterial Typing Techniques Cephalosporins Electrophoresis Gel Pulsed-Field Coliform bacteria DNA profiling Cattle Restriction fragment length polymorphism Ceftiofur Plasmids Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 78:4552-4560 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.00373-12 |
| Popis: | bla CTX-M beta-lactamases confer resistance to critically important cephalosporin drugs. Recovered from both hospital- and community-acquired infections, bla CTX-M was first reported in U.S. livestock in 2010. It has been hypothesized that veterinary use of cephalosporins in livestock populations may lead to the dissemination of beta-lactamase-encoding genes. Therefore, our objectives were to estimate the frequency and distribution of coliform bacteria harboring bla CTX-M in the fecal flora of Ohio dairy cattle populations. In addition, we characterized the CTX-M alleles carried by the isolates, their plasmidic contexts, and the genetic diversity of the bacterial isolates themselves. We also evaluated the association between ceftiofur use and the likelihood of recovering cephalosporinase-producing bacteria. Thirty fresh fecal samples and owner-reported ceftiofur use data were collected from each of 25 Ohio dairy farms. Fecal samples ( n = 747) yielded 70 bla CTX-M -positive Escherichia coli isolates from 5/25 herds, 715 bla CMY-2 E. coli isolates from 25/25 herds, and 274 Salmonella spp. from 20/25 herds. The within-herd prevalence among bla CTX-M -positive herds ranged from 3.3 to 100% of samples. Multiple pulsed-field gel electrophoresis (PFGE) patterns, plasmid replicon types, and CTX-M genes were detected. Plasmids with CTX-M-1, -15, and -14 alleles were clonal by restriction fragment length polymorphism (RFLP) within herds, and specific plasmid incompatibility group markers were consistently associated with each bla CTX-M allele. PFGE of total bacterial DNA showed similar within-herd clustering, with the exception of one herd, which revealed at least 6 different PFGE signatures. We were unable to detect an association between owner-reported ceftiofur use and the probability of recovering E. coli carrying bla CTX-M or bla CMY-2 . |
| Databáze: | OpenAIRE |
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