Diagnosis of tuberculosis infection using interferon-γ-based assays
| Autor: | Miguel Santín Cerezales, José Domínguez Benítez |
|---|---|
| Přispěvatelé: | Universitat de Barcelona |
| Rok vydání: | 2011 |
| Předmět: |
Microbiology (medical)
Risk Enzyme-Linked Immunospot Assay Tuberculosis Tuberculosi medicine.medical_treatment Interferó Enzyme-Linked Immunosorbent Assay Comorbidity Sensitivity and Specificity Vulnerable Populations Immunocompromised Host Interferon-gamma Interferon γ Tuberculosis diagnosis Diagnòstic Latent Tuberculosis Predictive Value of Tests Diagnosis medicine Humans T-SPOT.TB Aged business.industry Tuberculin Test ELISPOT Immunosuppression medicine.disease Prognosis bacterial infections and mycoses Body Fluids Occupational Diseases Predictive value of tests Tuberculosis Meningeal Immunology Interferon Contact Tracing business Contact tracing Biomarkers Forecasting |
| Zdroj: | Dipòsit Digital de la UB Universidad de Barcelona Recercat. Dipósit de la Recerca de Catalunya instname |
| Popis: | Interferon-γ-based assays, collectively known as IFN-γ release assays (IGRAs), have emerged as a reliable alternative to the old tuberculin skin test (TST) for the immunodiagnosis of tuberculosis (TB) infection. The 2 commercially available tests, the enzyme-linked immunosorbent assay (ELISA), QuantiFERON-TB Gold Intube (QFT-IT), and the enzyme-linked immunospot assay (ELISPOT), T-SPOT.TB, are more accurate than TST for the diagnosis of TB, since they are highly specific and correlate better with the existence of risk factors for the infection. According to the available data, T-SPOT.TB obtains a higher number of positive results than QFT-IT, while its specificity seems to be lower. Although the sensitivity of the IFN-γ -based assays may be impaired to some extent by cellular immunosuppression and extreme ages of life, they perform better than TST in these situations. Data from longitudinal studies suggest that IFN-γ-based tests are better predictors of subsequent development of active TB than TST; however this prognostic value has not been consistently demonstrated. This review focuses on the clinical use of the IFN-γ -based tests in different risk TB groups, and notes the main limitations and areas for future development. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|