Tumor ulceration, reduced infiltration of CD8-lymphocytes, high neutrophil-to-CD8-lymphocyte ratio and absence of MC virus are negative prognostic markers for patients with merkel cell carcinoma
| Autor: | Martin Heje, Rune Thomsen, Simon Naseri, Jeanette Bæhr Georgsen, Morten Ladekarl, Marie Louise Bønnelykke-Behrndtz, Torben Steiniche, Tine Engberg Damsgaard |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cancer Research Pathology medicine.medical_specialty Lymphocyte CD34 Merkel cell polyomavirus lcsh:RC254-282 Virus Article 03 medical and health sciences 0302 clinical medicine Merkel cell carcinoma medicine tumor microenvironment CD8 lymphocytes Ulceration Merkel cell polyoma virus integumentary system biology business.industry virus diseases food and beverages E-cadherin lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens biology.organism_classification medicine.disease ulceration 030104 developmental biology medicine.anatomical_structure Oncology Tumor microenvironment 030220 oncology & carcinogenesis Immunohistochemistry business Infiltration (medical) CD8 |
| Zdroj: | Naseri, S, Steiniche, T, Georgsen, J B, Thomsen, R, Ladekarl, M, Heje, M, Damsgaard, T E & Bønnelykke-Behrndtz, M L 2020, ' Tumor ulceration, reduced infiltration of CD8-lymphocytes, high neutrophil-to-CD8-lymphocyte ratio and absence of MC virus are negative prognostic markers for patients with merkel cell carcinoma ', Cancers, vol. 12, no. 4, 888 . https://doi.org/10.3390/cancers12040888 Cancers Volume 12 Issue 4 Naseri, S, Steiniche, T, Georgsen, J B, Thomsen, R, Ladekarl, M, Heje, M, Damsgaard, T E & Bønnelykke-Behrndtz, M L 2020, ' Tumor Ulceration, Reduced Infiltration of CD8-Lymphocytes, High Neutrophil-to-CD8-Lymphocyte Ratio and Absence of MC Virus are Negative Prognostic Markers for Patients with Merkel Cell Carcinoma ', Cancers, vol. 12, no. 4, 888, pp. 1-11 . https://doi.org/10.3390/cancers12040888 Cancers, Vol 12, Iss 888, p 888 (2020) |
| Popis: | (1) Background: Merkel cell carcinoma (MCC) is caused by the Merkel cell polyomavirus and UV radiation. Understanding of the underlying biology is limited, but identification of prognostic markers may lead to better prognostic stratification for the patients. (2) Methods: Ninety patients diagnosed with MCC (1996&ndash 2012) were included. Virus status was estimated by polymerase chain reaction (qPCR) and immunohistochemistry (IHC). Ulceration status, PD-L1, cd66b neutrophils, cd8 lymphocytes and biomarkers of vascularization (cd34 endothelial cells) and migration (e-cadherin) were estimated by IHC and analyzed with digital pathology. (3) Results: Virus was present in 47% of patient samples and correlated with lower E-cadherin expression (p = 0.0005), lower neutrophil-to-CD8 lymphocyte ratio (N:CD8 ratio) (p = 0.02) and increased PD-L1 expression (p = 0.03). Ulceration was associated with absence of virus (p = 0.03), increased neutrophil infiltration (p < 0.0001) and reduced CD8 lymphocyte infiltration (p = 0.04). In multivariate analysis, presence of virus (p = 0.01), ulceration (p = 0.05) and increased CD8 lymphocyte infiltration (p = 0.001) showed independent prognostic impacts on MCC-specific survival. (3) Conclusions: In this study, we found that a high N:CD8 ratio, ulceration, virus-negative status and absence of CD8 lymphocytes are negative prognostic markers. Accurate prognostic stratification of the patients may be important in the clinical setting for determination of adjuvant treatment. |
| Databáze: | OpenAIRE |
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