Prolactin regulates LAT1 expression via STAT5 (signal transducer and activator of transcription 5) signaling in mammary epithelial cells of dairy cows
| Autor: | Ying Shi, Minghui Jiang, Xiaoming Hou, Ye Lin, Shuyuan Song, Jinyu Zhou |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
endocrine system
Mammary gland Stimulation Large Neutral Amino Acid-Transporter 1 03 medical and health sciences Mammary Glands Animal Western blot STAT5 Transcription Factor Genetics medicine Animals Lactation Receptor STAT5 030304 developmental biology 0303 health sciences Gene knockdown biology medicine.diagnostic_test Chemistry 0402 animal and dairy science Caseins food and beverages Epithelial Cells 04 agricultural and veterinary sciences Milk Proteins 040201 dairy & animal science Prolactin Cell biology Milk medicine.anatomical_structure Protein Biosynthesis biology.protein STAT protein Cattle Female Animal Science and Zoology hormones hormone substitutes and hormone antagonists Signal Transduction Food Science |
| Zdroj: | Journal of Dairy Science. 103:6627-6634 |
| ISSN: | 0022-0302 |
| DOI: | 10.3168/jds.2019-17945 |
| Popis: | The l -type amino acid transporter 1 (LAT1; also known as SLC7A5) is a transporter that allows the uptake of large neutral amino acids into mammalian cells. In dairy cows, LAT1 is highly expressed in lactating mammary tissues and involved in milk protein synthesis. Prolactin (PRL) has a lactogenic role and is capable of inducing milk production in ruminants. However, the relationship between PRL stimulation and LAT1 expression in dairy cow mammary gland has not been well understood. In this study, we showed that PRL stimulation increased expression of LAT1 and β-casein in mammary epithelial cells of dairy cows. The stimulatory effect of PRL on milk protein production was inhibited by LAT1-specific inhibitor or LAT1 knockdown, suggesting that PRL-induced milk protein production is involved in LAT1 expression. To determine whether the PRL signaling pathway participates in regulation of LAT1 expression, PRLR (PRL receptor) or STAT5 (signal transducer and activator of transcription 5) was knocked down by short interfering (si)RNA in mammary epithelial cells of dairy cows. Western blot results showed that knockdown of PRLR or STAT5 with siRNA markedly decreased PRL-stimulated LAT1 expression. In addition, we observed a marked increase in plasma membrane expression of LAT1 in PRL-stimulated cells compared with control cells. These observations indicated that PRL signaling can regulate LAT1 expression and activity in mammary epithelial cells of dairy cows, contributing to increased amino acid availability and milk protein synthesis in mammary gland of dairy cow. |
| Databáze: | OpenAIRE |
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