Intracellular penetration of liposomes containing a water insoluble antimitotic drug in L1210 cells
| Autor: | Michel Deleers, Janos Fruhling, Willy Penasse, M. Vanhaelen, Jean Marie Ruysschaert, Christiane Brassinne, R. Vanhaelen-Fastre, Jerzy Hildebrand, G. Laurent |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Cytoplasm
Membrane Fluidity Cell Antineoplastic Agents Fluorescence Polarization Mice medicine Membrane fluidity Animals Leukemia L1210 Cell Nucleus Liposome Chemistry Nocodazole General Medicine Penetration (firestop) Microscopy Electron medicine.anatomical_structure Solubility Biochemistry Mice Inbred DBA Liposomes L1210 cells Benzimidazoles Carbamates Intracellular Fluorescence anisotropy |
| Zdroj: | European Journal of Cancer (1965). 16:1409-1416 |
| ISSN: | 0014-2964 |
| DOI: | 10.1016/0014-2964(80)90049-3 |
| Popis: | To demonstrate the possibility of cell delivery of water insoluble products, L1210 cells were incubated with liposomes containing Nocodazole® (NCL), an experimental drug totally insoluble in water. Liposomes of various lipid compositions readily incorporated NCL, but only those formed from dipalmitoyl phosphatidylcholine, cholesterol and stearylamine (molar ratio 4:3:1) had a clear affinity for L1210 cells, as shown by fluorescence studies. This affinity is mediated by the degree of both cell and liposome membrane fluidity. Cytoplasmic and intranuclear location of these liposomes containing NCL or drug-free and subcellular changes induced by their presence were demonstrated by ultrastructural studies. |
| Databáze: | OpenAIRE |
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